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FIGURE: 1 / 2
GNE Antibody (MA5-55459) in WB
Western Blot analysis of various lysates using GNE Recombinant Monoclonal Antibody (Product # MA5-55459) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55459
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5T9K10
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 623-722 of human GNE.
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: GALHLIQAAK LGNAKAQSIL RTAGTALGLG VVNILHTMNP SLVILSGVLA SHYIHIVKDV IRQQALSSVQ DVDVVVSDLV DPALLGAASM VLDYTTRRIY
Target Information
GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; N-acylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
Gene Aliases: 2310066H07Rik; DMRV; GLCNE; GNE; IBM2; NM; Uae1
UniProt ID: (Human) Q9Y223, (Rat) O35826, (Mouse) Q91WG8
Entrez Gene ID: (Human) 10020, (Rat) 114711, (Mouse) 50798
hydrolase activity, hydrolyzing O-glycosyl compounds
protein binding
ATP binding
UDP-N-acetylglucosamine 2-epimerase activity
N-acylmannosamine kinase activity
metal ion binding
nucleotide binding
catalytic activity
kinase activity
transferase activity
phosphotransferase activity, alcohol group as acceptor
hydrolase activity
DNA-binding transcription factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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