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Application
FIGURE: 1 / 1
GNL2 Antibody (PA5-89063) in WB
Western blot analysis of GNL2 in extracts of NIH/3T3 cells. Samples were incubated with GNL2 Polyclonal antibody (Product # PA5-89063) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89063
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GNL2 (NP_0374171)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.38 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805331
Product Specific Information
Immunogen sequence: MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQS SLQKFQEEMD TVMKDPYKVV MKQSKLPMSL LHDRIRPHNL KVHILDTESF ETTFGPKSQR KRPNLFASDM QSLIENAEMS TESYDQGKDR DLVTEDTGVR NEAQEEIYKK GQSKRIWGEL YKVIDSSDVV VQVLDARDPM GTRSPHIETY LKKEKPWKHL IFVLNKCDLV PTWATKRWVA VLSQDYPTLA FHASLTNPFG KGAFIQLLRQ; Positive Samples: NIH/3T3; Cellular Location: Nucleus, nucleolus
Target Information
GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation. May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed:26203195). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Autoantigen NGP-1; guanine nucleotide binding protein-like 2 (nucleolar); novel nucleolar guanosine 5'-triphosphate binding protein autoantigen; nucleolar G-protein gene 1; Nucleolar GTP-binding protein 2; nucleolar GTPase; nucleostemin-2; testicular antigen
Gene Aliases: BC003262; GNL2; HUMAUANTIG; Ngp-1; NGP1; Nog2; Nug2
UniProt ID: (Human) Q13823, (Mouse) Q99LH1
Entrez Gene ID: (Human) 29889, (Mouse) 230737
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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