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Proteintech

GPD2 Polyclonal Antibody

Name: GPD2 Polyclonal Antibody
Brand: Proteintech
SKU: 17219-1-AP
Price: 492.0 USD
Availability: InStock

12 References

View all (13) GPD2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (12)

Application

GPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

GPD2 Antibody (17219-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 17219-1-AP) (GPD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17219-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GPD2 Fusion Protein Ag11036 (5-378 aa encoded by BC019874)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KAVKGTLVG GGALATVLGL SQFAHYRRKQ MNLAYVKAAD CISEPVNREP PSREAQLLTL QNTSEFDILV IGGGATGSGC ALDAVTRGLK TALVERDDFS SGTSSRSTKL IHGGVRYLQK AIMKLDIEQY RMVKEALHER ANLLEIAPHL SAPLPIMLPV YKWWQLPYYW VGIKLYDLVA GSNCLKSSYV LSKSRALEHF PMLQKDKLVG AIVYYDGQHN DARMNLAIAL TAARYGAATA NYMEVVSLLK KTDPQTGKVH VSGARCKDVL TGQEFDVRAK CVINATGPFT DSVRKMDDKD AAAICQPSAG VHIVMPGYYS PESMGLLDPA TSDGRVIFFL PWQKMTIAGT TDTPTDVTHH PIPSEEDINF ILNEV (5-378 aa encoded by BC019874)

Target Information

Mitochondrial glycerophosphate dehydrogenase (EC 1. 1. 99. 5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis.

Molecular cell

Yao CH,Park JS,Kurmi K,Hu SH,Notarangelo G,Crowley J,Jacobson H,Hui S,Sharpe AH,Haigis MC

Thu Apr 20 00:00:00 UTC 2023

Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.

Journal of virology

Liu C,Zhao K,Chen Y,Yao Y,Tang J,Wang J,Xu C,Yang Q,Zheng Y,Yuan Y,Sun H,Zhang Y,Zhou Y,Chen J,Wang Y,Wu C,Pei R,Chen X

Wed May 31 00:00:00 UTC 2023

Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.

Journal of virology

Liu C,Zhao K,Chen Y,Yao Y,Tang J,Wang J,Xu C,Yang Q,Zheng Y,Yuan Y,Sun H,Zhang Y,Zhou Y,Chen J,Wang Y,Wu C,Pei R,Chen X

Wed May 31 00:00:00 UTC 2023

A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.

Proceedings of the National Academy of Sciences of the United States of America

Wu S,Mao C,Kondiparthi L,Poyurovsky MV,Olszewski K,Gan B

Tue Jun 28 00:00:00 UTC 2022

Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD⁺ to alleviate mitochondrial disease.

Cell metabolism

Liu S,Fu S,Wang G,Cao Y,Li L,Li X,Yang J,Li N,Shan Y,Cao Y,Ma Y,Dong M,Liu Q,Jiang H

Tue Oct 05 00:00:00 UTC 2021

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M,Friedman J,Jost M,Xu J,Yamada J,Kampmann M,Horlbeck MA,Salemi MR,Phinney BS,Weissman JS,Nunnari J

Wed May 26 00:00:00 UTC 2021

Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.

The Journal of biological chemistry

Alshawi A,Agius L

Fri Feb 22 00:00:00 UTC 2019

Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.

The Journal of biological chemistry

Alshawi A,Agius L

Fri Feb 22 00:00:00 UTC 2019

Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.

Nature immunology

Langston PK,Nambu A,Jung J,Shibata M,Aksoylar HI,Lei J,Xu P,Doan MT,Jiang H,MacArthur MR,Gao X,Kong Y,Chouchani ET,Locasale JW,Snyder NW,Horng T

Sun Sep 01 00:00:00 UTC 2019

Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.

FEBS letters

Garcia J,Decker CW,Sanchez SJ,Ouk JM,Siu KM,Han D

Thu Mar 01 00:00:00 UTC 2018

Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.

FEBS letters

Garcia J,Decker CW,Sanchez SJ,Ouk JM,Siu KM,Han D

Thu Mar 01 00:00:00 UTC 2018

Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.

Free radical biology & medicine

Han D,Johnson HS,Rao MP,Martin G,Sancheti H,Silkwood KH,Decker CW,Nguyen KT,Casian JG,Cadenas E,Kaplowitz N

Sun Jan 01 00:00:00 UTC 2017

Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.

Free radical biology & medicine

Han D,Johnson HS,Rao MP,Martin G,Sancheti H,Silkwood KH,Decker CW,Nguyen KT,Casian JG,Cadenas E,Kaplowitz N

Sun Jan 01 00:00:00 UTC 2017

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.

European journal of human genetics : EJHG

Joshi M,Eagan J,Desai NK,Newton SA,Towne MC,Marinakis NS,Esteves KM,De Ferranti S,Bennett MJ,McIntyre A,Beggs AH,Berry GT,Agrawal PB

Wed Oct 01 00:00:00 UTC 2014

A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.

European journal of human genetics : EJHG

Joshi M,Eagan J,Desai NK,Newton SA,Towne MC,Marinakis NS,Esteves KM,De Ferranti S,Bennett MJ,McIntyre A,Beggs AH,Berry GT,Agrawal PB

Wed Oct 01 00:00:00 UTC 2014

A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.

The Journal of biological chemistry

Orr AL,Quinlan CL,Perevoshchikova IV,Brand MD

Fri Dec 14 00:00:00 UTC 2012

A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.

The Journal of biological chemistry

Orr AL,Quinlan CL,Perevoshchikova IV,Brand MD

Fri Dec 14 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: FAD-linked glycerol-3-phosphate dehydrogenase; glycerol phosphate dehydrogenase 1, mitochondrial; Glycerol-3-phosophate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2, mitochondrial; Glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; GPDH-M; mGDH; mitochondrial glycerol-3-phosphate dehydrogenase; mitochondrial glycerophosphate dehydrogenase; mitohondrial glycerophosphate dehydrogenase gene; mtGPD; mtGPDH gene, promoter region and alternative transcripts; Protein TISP38; testicular tissue protein Li 76

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Gene Aliases: AA408484; AI448216; AU021455; AW494132; GDH2; Gdm1; Gpd-m; GPD2; GPDH; Gpdh-m; GPDM; mGPDH; mtGPDH; TISP38

UniProt ID: (Human) P43304, (Mouse) Q64521, (Rat) P35571

Entrez Gene ID: (Human) 2820, (Mouse) 14571, (Rat) 25062

Function(s)

glycerol-3-phosphate dehydrogenase activity calcium ion binding sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity glycerol-3-phosphate dehydrogenase [NAD+] activity oxidoreductase activity metal ion binding sn-glycerol-3-phosphate:ubiquinone oxidoreductase activity dehydrogenase

Process(es)

glycerol-3-phosphate metabolic process gluconeogenesis glycerol catabolic process multicellular organism growth camera-type eye development cellular lipid metabolic process oxidation-reduction process NADH metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GPD2 Polyclonal Antibody

GPD2 Antibody (17219-1-AP) in IHC (P)

Product Details

17219-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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