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Invitrogen
GPLD1 Polyclonal Antibody
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Application
FIGURE: 1 / 1
GPLD1 Antibody (PA5-75632) in WB
Western blot analysis of GPLD1 in Lane 1: A549 whole cell lysate, Lane 2: mouse brain tissue lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with GPLD1 polyclonal antibody (Product # PA5-75632) at a dilution of 1:500.
Product Details
PA5-75632
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues in Human GPLD1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2719360
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with polipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPLD1, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; glycosylphosphatidylinositol phospholipase D; glycosylphosphatidylinositol specific phospholipase D1, isoform 2; GPI-PLD; GPI-specific phospholipase D; MGC22590; Phosphatidylinositol-glycan-specific phospholipase D; PI-G PLD
Gene Aliases: 6330541J12Rik; AW546131; GPIPLD; GPIPLDM; GPLD1; PIGPLD; PIGPLD1; PLD
UniProt ID: (Human) P80108, (Rat) Q8R2H5
Entrez Gene ID: (Human) 2822, (Rat) 291132, (Mouse) 14756
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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