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Alomone Labs, LTD
GPR65 (TDAG8) (extracellular) Polyclonal Antibody, FITC
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Application
FIGURE: 1 / 2
GPR65 (TDAG8) (extracellular) Antibody (AGR-043-F-50UL) in Flow
Cell surface detection of GPR65 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + rabbit IgG isotype control-FITC. (green) Cells + Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody (#AGR-043-F), (5 µg).
Product Details
AGR-043-F-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DYTWNKDNWTFSP, corresponding to amino acid residues 72-84 of human GPR65, 1st extracellular loop
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
GPR65 also known as TDAG8 is a transmembrane protein belonging to the G protein coupled receptor super family with 337-amino acid and 90% similarity and 81% identity in the 7 transmembrane domains of its mouse homolog. It has 3 potential N-linked glycosylation sites and is expressed predominantly in lymphoid organs and cancer tissues with increased expression during activation-induced death of T-cell hybridomas. Two major transcripts of 4.5 kb and 1.8 kb are found in peripheral blood leukocytes and functions as a novel proton-sensing receptor. It couples to adenylyl cyclase and psychosine and is also actively involved in the establishment of immunological autotolerance. GPR65 has a role in cellular transformation by RhoA activation and actin rearrangement under conditions accompanying tissue acidosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 25; G-protein coupled receptor 65; Psychosine receptor; T cell death associated protein 8; T-cell death-associated gene 8 protein
Gene Aliases: Dig1; Gpcr25; GPR65; hTDAG8; TDAG8
UniProt ID: (Human) Q8IYL9, (Mouse) Q61038
Entrez Gene ID: (Human) 8477, (Rat) 299242, (Mouse) 14744
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