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Application
FIGURE: 1 / 4
GPRC5B Antibody (PA5-106843) in ICC/IF
Immunocytochemistry analysis of GPRC5B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GPRC5B polyclonal antibody (Product # PA5-106843) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GPRC5B(Accession Q9NZH0), corresponding to amino acid residues A61-F111.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854507
Product Specific Information
Antibody detects endogenous levels of total GPRC5B.
Target Information
This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-69G12.1; G protein-coupled receptor, class C, group 5, member B; G protein-coupled receptor, family C, group 1, member B; G-protein coupled receptor family C group 5 member B; RAIG-2; retinoic acid responsive gene protein; Retinoic acid-induced gene 2 protein
Gene Aliases: GPRC5B; RAIG-2; RAIG2
UniProt ID: (Human) Q9NZH0
Entrez Gene ID: (Human) 51704
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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