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Application
FIGURE: 1 / 2
GREM2 Antibody (PA5-119878) in WB
Western blot analysis of PRDC on CAL62 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. GREM2 Polyclonal Antibody (Product # PA5-119878) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Product Details
PA5-119878
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human PRDC aa 50-100
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913450
Product Specific Information
Positive Control: CAL62 cell lysates, SH-SY5Y.
Subcellular Location: Secreted.
Target Information
GREM2 encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3Prdc; gremlin 2, cysteine knot superfamily, homolog; Gremlin-2; PRDCgremlin 2; Protein related to DAN and cerberus
Gene Aliases: CKTSF1B2; DAND3; GREM2; PRDC
UniProt ID: (Human) Q9H772
Entrez Gene ID: (Human) 64388
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