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Application
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GRIK5 (GluK5) (extracellular) Antibody (AGC-042-200UL) in IHC (F)
Expression of kainate receptor GluK5 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:300), followed by goat- Anti-rabbit-Alexa-488. GluK5 staining (green)appears in neurons (horizontal arrows) of the pyramidal layer (Pyr) and in apical dendrites (vertical arrows) of mouse hippocampus. Nuclei a... View More
Product Details
AGC-042-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5, Extracellular, N-terminus
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik5, also known as kainate-preferring glutamate recptor subunit KA2, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik5 is highly homologous to the related ionotrophic glutamate receptor Grik4 (also known as KA1). Like Grik4, Grik5 does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAA2; Excitatory amino acid receptor 2; GluK5; GluR gamma-2; glutamate receptor; Glutamate receptor gamma-2; Glutamate receptor ionotropic, kainate 5; Glutamate receptor KA-2; glutamate receptor KA2; glutamate receptor, ionotropic kainate 5; glutamate receptor, ionotropic, kainate 5; KA2
Gene Aliases: EAA2; GluK5; GluRgamma2; GRIK2; GRIK5; iGlu5; KA2
UniProt ID: (Human) Q16478, (Rat) Q63273, (Mouse) Q61626
Entrez Gene ID: (Human) 2901, (Rat) 24407, (Mouse) 14809
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