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Invitrogen
GRK2 Recombinant Rabbit Monoclonal Antibody (ST05-60)
Product Details
MA5-32181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ST05-60
Immunogen
Synthetic peptide within Human GRK2 aa 640-689
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809469
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; Beta-ARK-1; EC 2.7.1.126; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2
Gene Aliases: Adrbk-1; ADRBK1; BARK; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK-2; GRK2
UniProt ID: (Mouse) Q99LL8, (Rat) P26817, (Human) P25098
Entrez Gene ID: (Mouse) 110355, (Rat) 25238, (Human) 156
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
G-protein coupled receptor kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Edg-2 lysophosphatidic acid receptor binding
beta-adrenergic receptor kinase activity
alpha-2A adrenergic receptor binding
non-receptor serine/threonine protein kinase
regulation of the force of heart contraction
desensitization of G-protein coupled receptor protein signaling pathway
negative regulation of the force of heart contraction by chemical signal
protein phosphorylation
response to oxidative stress
signal transduction
G-protein coupled receptor signaling pathway
tachykinin receptor signaling pathway
heart development
response to organic cyclic compound
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
viral genome replication
receptor internalization
positive regulation of catecholamine secretion
termination of G-protein coupled receptor signaling pathway
follicle-stimulating hormone signaling pathway
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of striated muscle contraction
viral entry into host cell
cardiac muscle contraction
aging
response to hydrogen peroxide
G-protein coupled acetylcholine receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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