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Application
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GRK3 Antibody (MA5-42913) in WB
Western blot analysis of GRK3 in extracts of various cell lines. Samples were incubated with GRK3 Monoclonal antibody (Product # MA5-42913) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-42913
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10O6S3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 589-688 of human GRK3 (P35626).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912054
Product Specific Information
Positive test controls include: Jurkat, SH-SY5Y.
Immunogen sequence: EWRGEGESRQ NLLTMEQILS VEETQIKDKK CILFRIKGGK QFVLQCESDP EFVQWKKELN ETFKEAQRLL RRAPKFLNKP RSGTVELPKP SLCHRNSNGL
Target Information
ADRBK2, or adrenergic beta receptor kinase 2 is a serine/threonine kinase that is thought to act as a regulator of receptor function. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic, beta, receptor kinase 2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G protein-coupled receptor kinase 3; G-protein-coupled receptor kinase 3
Gene Aliases: ADRBK2; BARK2; GRK3
UniProt ID: (Human) P35626
Entrez Gene ID: (Human) 157
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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