Loading ...

Proteintech

GRP94 Polyclonal Antibody

6 References

View all (40) GRP94 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (12)

Application

GRP94 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

GRP94 Antibody (10979-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 10979-1-AP (GRP94 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

GRP94 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GRP94 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10979-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GRP94 Fusion Protein Ag1439 (1-315 aa encoded by BC009195)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was generated against the N-terminal region of full-length HSP90b1.

Immunogen sequence: MRALWVLGL CCVLLTFGSV RADDEVDVDG TVEEDLGKSR EGSRTDDEVV QREEEAIQLD GLNASQIREL REKSEKFAFQ AEVNRMMKLI INSLYKNKEI FLRELISNAS DALDKIRLIS LTDENALSGN EELTVKIKCD KEKNLLHVTD TGVGMTREEL VKNLGTIAKS GTSEFLNKMT EAQEDGQSTS ELIGQFGVGF YSAFLVADKV IVTSKHNNDT QHIWESDSNE FSVIADPRGN TLGRGTTITL VLKEEASDYL ELDTIKNLVK KYSQFINFPI YVWSSKTETV EEPMEEEEAA KEEKEESDDE AAARRR (1-315 aa encoded by B C009195)

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Journal of neuroinflammation

Liu N,Bai L,Lu Z,Gu R,Zhao D,Yan F,Bai J

Sat Jan 29 00:00:00 UTC 2022

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis.

Journal of immunology research

Hong Q,Zhang Y,Lin W,Wang W,Yuan Y,Lin J,Xie Z,Li X,Meng Y

Tue Aug 30 00:00:00 UTC 2022

Journal of immunology research

Hong Q,Zhang Y,Lin W,Wang W,Yuan Y,Lin J,Xie Z,Li X,Meng Y

Tue Aug 30 00:00:00 UTC 2022

Journal of immunology research

Hong Q,Zhang Y,Lin W,Wang W,Yuan Y,Lin J,Xie Z,Li X,Meng Y

Tue Aug 30 00:00:00 UTC 2022

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Veterinary microbiology

Zhang L,Jin M,Song M,Liu S,Wang T,Guo K,Zhang Y

Thu Apr 01 00:00:00 UTC 2021

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Molecular medicine reports

Liu M,Liu S,Tan W,Tang F,Long J,Li Z,Liang B,Chu C,Yang J

Fri Dec 01 00:00:00 UTC 2017

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Molecular medicine reports

Liu M,Liu S,Tan W,Tang F,Long J,Li Z,Liang B,Chu C,Yang J

Fri Dec 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family C member 4; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96

View more View less

Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1

UniProt ID: (Human) P14625, (Mouse) P08113, (Rat) Q66HD0

Entrez Gene ID: (Human) 7184, (Mouse) 22027, (Rat) 362862

Function(s)

RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding nucleotide binding Hsp90 family chaperone

Process(es)

response to hypoxia toll-like receptor signaling pathway receptor-mediated endocytosis protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ATF6-mediated unfolded protein response negative regulation of apoptotic process regulation of phosphoprotein phosphatase activity sequestering of calcium ion cellular response to ATP protein folding response to stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GRP94 Polyclonal Antibody

GRP94 Antibody (10979-1-AP) in ICC/IF

Product Details

10979-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!