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            • Primary Antibodies ›
            • GRSF1 Antibodies

            Bethyl Laboratories

            GRSF1 Polyclonal Antibody

            View all (13) GRSF1 antibodies
            Datasheet
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            Catalog # A305-137A
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            Price (EUR)
            445,00
             
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            Cite GRSF1 Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            GRSF1 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            GRSF1 Antibody (A305-137A) in WB

            Detection of human GRSF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GRSF1 antibody (Product # A305-137A) (lot A305-137A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
            View Product

            GRSF1 Antibody in Western Blot (WB)
            GRSF1 Antibody in Immunoprecipitation (IP)

            Product Details

            A305-137A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-10 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 325 to 375 of human G-rich sequence factor 1.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            phosphate/tris citrate, pH 7-8

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Target Information

            G-rich sequence factor 1 (GRSF1) is a regulator of post-transcriptional mitochondrial gene expression. It required for assembly of the mitochondrial ribosome and for recruitment of mRNS and IncRNA. GRSF1 binds RNAs containing the 14 base G-rich element. It preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-NDS - each of which contains multiple consensus binding sequences.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: G-rich sequence factor 1; GRSF-1

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            Gene Aliases: GRSF1

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            UniProt ID: (Human) Q12849

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            Entrez Gene ID: (Human) 2926

            View more View less

            Function(s)

            nucleotide binding RNA binding mRNA binding poly(A) RNA binding RNA splicing factor

            Process(es)

            mRNA polyadenylation tRNA processing anterior/posterior pattern specification morphogenesis of embryonic epithelium
            It has to be done as per old AB suggested Products section.
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