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            • Primary Antibodies ›
            • GSS Antibodies

            Invitrogen

            GSS Recombinant Rabbit Monoclonal Antibody (JB95-33)

            View all (29) GSS antibodies
            Datasheet
            Protocols
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            Datasheet
            Protocols
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            Cite GSS Recombinant Rabbit Monoclonal Antibody (JB95-33)

            • Antibody Testing Data (8)
            Application
            GSS Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 8

            GSS Antibody (MA5-34698) in ICC/IF

            Immunofluorescent analysis of Glutathione Synthetase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), followed by DAPI (blue).
            View Product

            GSS Antibody in Immunocytochemistry (ICC/IF)
            GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            GSS Antibody in Western Blot (WB)
            GSS Antibody in Flow Cytometry (Flow)

            Product Details

            MA5-34698

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:1,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            -

            Immunocytochemistry (ICC/IF)

            1:50-1:200
            -

            Flow Cytometry (Flow)

            1:50-1:100
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Expression System

            HEK293 cells

            Class

            Recombinant Monoclonal

            Type

            Antibody

            Clone

            JB95-33

            Immunogen

            Recombinant protein within Human Glutathione Synthetase aa 301-420
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein A

            Storage buffer

            TBS, pH 7.4, with 40% glycerol, 0.05% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles, store in dark

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2848606

            Product Specific Information

            Positive Control: SiHa, mouse colon tissue, rat liver tissue lysate, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, HepG2, LOVO.

            Target Information

            Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB; MGC14098; OTTHUMP00000030711

            View more View less

            Gene Aliases: GSHS; GSS; HEL-S-64p; HEL-S-88n

            View more View less

            UniProt ID: (Human) P48637

            View more View less

            Entrez Gene ID: (Human) 2937

            View more View less

            Function(s)

            magnesium ion binding glutathione synthase activity protein binding ATP binding glycine binding protein homodimerization activity glutathione binding ligase

            Process(es)

            cellular amino acid metabolic process glutathione biosynthetic process response to oxidative stress nervous system development aging response to xenobiotic stimulus response to nutrient levels response to tumor necrosis factor response to amino acid response to cadmium ion
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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