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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • GST Antibodies

            Invitrogen

            GST Tag Monoclonal Antibody (8-326)

            Advanced Verification
            21 Published Figures
            57 References
            View all (102) GST antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite GST Tag Monoclonal Antibody (8-326)

            • Antibody Testing Data (4)
            • Published Figures (21)
            • Advanced Verification (2)
            Application
            GST Tag Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 27

            GST Tag Antibody (MA4-004) in WB

            Western blot was performed using GST Tag Monoclonal Antibody (8-326) (Product # MA4-004) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-GST, 50 µg (Lane 3), H3-GST, 25 µg (Lane 4), H3-GST, 12.5 µg (Lane 5), H3-GST, 6.25 µg (Lane 6), GST-p65, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB... View More
            View Product

            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Immunoprecipitation (IP)
            GST Tag Antibody in Immunoprecipitation (IP)
            GST Tag Antibody in Immunocytochemistry (ICC/IF)
            GST Tag Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody in Western Blot (WB)
            GST Tag Antibody
            GST Tag Antibody

            Product Details

            MA4-004

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            View 45 publications 45 publications

            Immunocytochemistry (ICC/IF)

            -
            View 4 publications 4 publications

            ELISA (ELISA)

            -
            View 2 publications 2 publications

            Immunoprecipitation (IP)

            2 µg
            View 4 publications 4 publications

            In vitro Assay (IV)

            -
            View 1 publication 1 publication

            SDS-PAGE (SDS-PAGE)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Tag

            Published species

            Tag

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            8-326

            Immunogen

            Recombinant purified full-length GST

            Conjugate

            Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 555 
            • Alexa Fluor 647 
            • Biotin 
            • DyLight 680
            • DyLight 800 4X PEG
            • HRP
            • Request custom conjugation

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein G

            Storage buffer

            PBS with 1mg/mL BSA

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10979611

            Target Information

            GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
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            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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