Invitrogen

Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

16 References

View all (29) GATA4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

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Gata-4 Antibody (14-9980-82) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Ovary (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-GATA-4 Rat Monoclonal Antibody (Product # 14-9980-82, 1:1000 dilution) and detected by chemiluminescence using F(ab"e)2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to GATA-4 was detected across the tissues tested. Additional band around 25kDa from circulating IgGs was observed in Mo... View More

Gata-4 Antibody in Immunocytochemistry (ICC/IF)

Gata-4 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

Gata-4 Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

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Product Details

14-9980-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioEvan

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763541

Product Specific Information

Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4.

Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48 kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.

Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry.

Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2 µg/mL for western blot analysis. The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generating human blastoids modeling blastocyst-stage embryos and implantation.

Nature protocols

Heidari Khoei H,Javali A,Kagawa H,Sommer TM,Sestini G,David L,Slovakova J,Novatchkova M,Scholte Op Reimer Y,Rivron N

14-9980-82 was used in Immunocytochemistry to describe how we formed human blastoids that (1) efficiently achieve the morphology of the blastocyst and (2) form lineages according to the pace and sequence of blastocyst development, (3) ultimately forming cells that transcriptionally reflect the blastocyst (preimplantation stage).

Mon May 01 00:00:00 EDT 2023

Ascl1 and Ngn2 convert mouse embryonic stem cells to neurons via functionally distinct paths.

Nature communications

Vainorius G,Novatchkova M,Michlits G,Baar JC,Raupach C,Lee J,Yelagandula R,Wernig M,Elling U

14-9980-82 was used in Immunocytochemistry to highlight that cell type conversion can occur through two distinct mechanistic paths, even when induced by closely related transcription factors.

Sat Sep 02 00:00:00 EDT 2023

Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells.

Cell stem cell

Pham TXA,Panda A,Kagawa H,To SK,Ertekin C,Georgolopoulos G,van Knippenberg SSFA,Allsop RN,Bruneau A,Chui JS,Vanheer L,Janiszewski A,Chappell J,Oberhuemer M,Tchinda RS,Talon I,Khodeer S,Rossant J,Lluis F,David L,Rivron N,Balaton BP,Pasque V

14-9980-82 was used in Immunohistochemistry (PFA) to investigate the mechanisms involved in differentiation of naive human pluripotent stem cells into EXM cells.

Thu Sep 01 00:00:00 EDT 2022

Naive stem cell blastocyst model captures human embryo lineage segregation.

Cell stem cell

Yanagida A,Spindlow D,Nichols J,Dattani A,Smith A,Guo G

Published figure using Gata-4 monoclonal antibody (Product # 14-9980-82) in Immunocytochemistry

Thu Jun 03 00:00:00 EDT 2021

Naive stem cell blastocyst model captures human embryo lineage segregation.

Cell stem cell

Yanagida A,Spindlow D,Nichols J,Dattani A,Smith A,Guo G

Published figure using Gata-4 monoclonal antibody (Product # 14-9980-82) in Immunocytochemistry

Thu Jun 03 00:00:00 EDT 2021

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Cardiovascular diabetology

Cohen CD,De Blasio MJ,Lee MKS,Farrugia GE,Prakoso D,Krstevski C,Deo M,Donner DG,Kiriazis H,Flynn MC,Gaynor TL,Murphy AJ,Drummond GR,Pinto AR,Ritchie RH

14-9980-82 was used in Immunohistochemistry and Immunocytochemistry to characterise the changes in the cardiac cellular landscape in murine diabetes to identify potential cellular protagonists in the diabetic heart.

Tue Jun 01 00:00:00 EDT 2021

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Cardiovascular diabetology

Cohen CD,De Blasio MJ,Lee MKS,Farrugia GE,Prakoso D,Krstevski C,Deo M,Donner DG,Kiriazis H,Flynn MC,Gaynor TL,Murphy AJ,Drummond GR,Pinto AR,Ritchie RH

Tue Jun 01 00:00:00 EDT 2021

Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.

Frontiers in physiology

Koch PS,Sandorski K,Heil J,Schmid CD,Kürschner SW,Hoffmann J,Winkler M,Staniczek T,de la Torre C,Sticht C,Schledzewski K,Taketo MM,Trogisch FA,Heineke J,Géraud C,Goerdt S,Olsavszky V

Published figure using Gata-4 monoclonal antibody (Product # 14-9980-82) in Immunocytochemistry

Fri Oct 22 00:00:00 EDT 2021

Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.

Frontiers in physiology

Koch PS,Sandorski K,Heil J,Schmid CD,Kürschner SW,Hoffmann J,Winkler M,Staniczek T,de la Torre C,Sticht C,Schledzewski K,Taketo MM,Trogisch FA,Heineke J,Géraud C,Goerdt S,Olsavszky V

Published figure using Gata-4 monoclonal antibody (Product # 14-9980-82) in Immunocytochemistry

Fri Oct 22 00:00:00 EDT 2021

Modeling High-Grade Serous Ovarian Carcinoma Using a Combination of In Vivo Fallopian Tube Electroporation and CRISPR-Cas9-Mediated Genome Editing.

Cancer research

Teng K,Ford MJ,Harwalkar K,Li Y,Pacis AS,Farnell D,Yamanaka N,Wang YC,Badescu D,Ton Nu TN,Ragoussis J,Huntsman DG,Arseneau J,Yamanaka Y

14-9980-82 was used in Immunohistochemistry (Paraffin), Western Blot to generate genetic mouse models of ovarian cancer with high flexibility in selecting mutation combinations and targeting areas.

Fri Oct 15 00:00:00 EDT 2021

Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation.

Scientific reports

Sarrach S,Huang Y,Niedermeyer S,Hachmeister M,Fischer L,Gille S,Pan M,Mack B,Kranz G,Libl D,Merl-Pham J,Hauck SM,Paoluzzi Tomada E,Kieslinger M,Jeremias I,Scialdone A,Gires O

14-9980 was used in Flow cytometry/Cell sorting to comprehensively describe the timing and rationale of EpCAM regulation in early murine gastrulation and embryonic stem cell differentiation.

Mon Jan 29 00:00:00 EST 2018

Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation.

Scientific reports

Sarrach S,Huang Y,Niedermeyer S,Hachmeister M,Fischer L,Gille S,Pan M,Mack B,Kranz G,Libl D,Merl-Pham J,Hauck SM,Paoluzzi Tomada E,Kieslinger M,Jeremias I,Scialdone A,Gires O

14-9980 was used in Flow cytometry/Cell sorting to comprehensively describe the timing and rationale of EpCAM regulation in early murine gastrulation and embryonic stem cell differentiation.

Mon Jan 29 00:00:00 EST 2018

Genetic Intersection of Tsix and Hedgehog Signaling during the Initiation of X-Chromosome Inactivation.

Developmental cell

Del Rosario BC,Del Rosario AM,Anselmo A,Wang PI,Sadreyev RI,Lee JT

14-9980 was used in Immunocytochemistry-immunoflourescence to study the genetic Intersection of Tsix and Hedgehog signalling during the initiation of X-chromosome inactivation.

Mon Nov 06 00:00:00 EST 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

14998082 was used in immunocytochemistry to investigate the gene expression dynamics of early mouse embryonic stem cell differentiation

Wed Mar 15 00:00:00 EDT 2017

CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.

Toxicology letters

Pollack D,Xiao Y,Shrivasatava V,Levy A,Andrusier M,D'Armiento J,Holz MK,Vigodner M

14-9980 was used in Immunocytochemistry-immunoflourescence to examine the gene expression patterns in the testis of mice exposed to tobacco smoke.

Thu Apr 16 00:00:00 EDT 2015

Overexpression of aromatase associated with loss of heterozygosity of the STK11 gene accounts for prepubertal gynecomastia in boys with Peutz-Jeghers syndrome.

The Journal of clinical endocrinology and metabolism

Ham S,Meachem SJ,Choong CS,Charles AK,Baynam GS,Jones TW,Samarajeewa NU,Simpson ER,Brown KA

14-9980 was used in Immunohistochemistry to characterise the role of LKB1 in regulating aromatase expression in the testis of two boys with Peutz-Jeghers syndrome.

Sun Dec 01 00:00:00 EST 2013

Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.

Spermatogenesis

Nicholls PK,Stanton PG,Rainczuk KE,Qian H,Gregorevic P,Harrison CA

14-9980 was used in Immunocytochemistry to describe a modified inoculation and centrifugation protocol to improve Sertoli cell transduction in vitro.

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4

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Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369

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Entrez Gene ID: (Human) 2626, (Mouse) 14463

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding metal ion binding DNA-binding transcription factor

Process(es)

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis atrioventricular valve formation mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis heart development regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation atrioventricular canal development response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development cardiac muscle cell differentiation Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™

Gata-4 Antibody (14-9980-82) in WB

Product Details

14-9980-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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