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Application
FIGURE: 1 / 1
Gliadin Antibody (PA5-97536) in WB
Western Blot analysis of Gliadin using a Gliadin Polyclonal antibody (Product # PA5-97536) at a concentration of 2 µg/mL. Lane 1: Wheat flour at 2µg. Lane 2: Wheat flour at 10µg. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:15,000 dilution. Observed band size: 35 kDa.
Product Details
PA5-97536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Wheat Gliadin (Native Protein)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2812152
Target Information
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGP; gliadin peptide
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