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            • Primary Antibodies ›
            • Glucocorticoid Receptor (Nr3c1) Antibodies

            Proteintech

            Glucocorticoid receptor Polyclonal Antibody

            95 References
            View all (66) Glucocorticoid Receptor (Nr3c1) antibodies

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            Datasheet
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            Catalog # 24050-1-AP
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            Price (USD)
            455.00
            150 µL
             
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            Datasheet
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            Cite Glucocorticoid receptor Polyclonal Antibody

            • Antibody Testing Data (11)
            Application
            Glucocorticoid receptor Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 11

            Glucocorticoid receptor Antibody (24050-1-AP) in ICC/IF

            Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (Product # 24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
            View Product

            Glucocorticoid receptor Antibody in Immunocytochemistry (ICC/IF)
            Glucocorticoid receptor Antibody in Immunocytochemistry (ICC/IF)
            Glucocorticoid receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Western Blot (WB)
            Glucocorticoid receptor Antibody in Immunoprecipitation (IP)

            Product Details

            24050-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:16,000
            View 56 publications 56 publications

            Immunohistochemistry (IHC)

            -
            View 3 publications 3 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:200
            -

            Immunocytochemistry (ICC/IF)

            1:20-1:200
            View 14 publications 14 publications

            Immunoprecipitation (IP)

            0.5-4.0 µg
            View 7 publications 7 publications

            ChIP assay (ChIP)

            Assay-dependent
            View 15 publications 15 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Published species

            Fish, Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Glucocorticoid receptor Fusion Protein Ag21146 (1-350 aa encoded by BC015610)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.43 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            This antibody is specific to NR3C1. NR3C1 exists 16 isoforms and range of molecular weight is 54-94 kDa.

            Immunogen sequence: MDSKESLTP GREENPSSVL AQERGDVMDF YKTLRGGATV KVSASSPSLA VASQSDSKQR RLLVDFPKGS VSNAQQPDLS KAVSLSMGLY MGETETKVMG NDLGFPQQGQ ISLSSGETDL KLLEESIANL NRSTSVPENP KSSASTAVSA APTEKEFPKT HSDVSSEQQH LKGQTGTNGG NVKLYTTDQS TFDILQDLEF SSGSPGKETN ESPWRSDLLI DENCLLSPLA GEDDSFLLEG NSNEDCKPLI LPDTKPKIKD NGDLVLSSPS NVTLPQVKTE KEDFIELCTP GVIKQEKLGT VYCQASFPGA NIIGNKMSAI SVHGVSTSGG QMYHYDMNTA SLSQQQDQKP I (1-350 aa encoded by BC015610)

            Target Information

            Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858

            View more View less

            Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1

            View more View less

            UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536

            View more View less

            Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413

            View more View less

            Function(s)

            RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity steroid hormone binding RNA polymerase II regulatory region DNA binding DNA binding chromatin binding double-stranded DNA binding steroid hormone receptor activity transcription coactivator activity transcription factor binding lipid binding Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding hormone binding protein homodimerization activity sequence-specific DNA binding metal ion binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding

            Process(es)

            regulation of gluconeogenesis chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glucocorticoid metabolic process regulation of glucose metabolic process positive regulation of glutamate secretion chromatin modification adrenal gland development negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of vascular permeability steroid hormone mediated signaling pathway glucocorticoid mediated signaling pathway positive regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance chromatin-mediated maintenance of transcription mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus negative regulation of glucocorticoid mediated signaling pathway positive regulation of glucocorticoid receptor signaling pathway brain development aging circadian rhythm androgen metabolic process response to radiation response to activity response to inactivity muscle atrophy lung development response to insulin response to mercury ion response to corticosterone response to calcium ion response to electrical stimulus cellular response to magnesium ion response to dexamethasone transcription initiation from RNA polymerase II promoter apoptotic process chromosome segregation mitotic nuclear division signal transduction covalent chromatin modification cell division
            It has to be done as per old AB suggested Products section.
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