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            • Primary Antibodies ›
            • GAPDH Antibodies

            Nordic

            Glyceraldehyde-3-phosphate dehydrogenase Polyclonal Antibody

            View all (122) GAPDH antibodies
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            Catalog # NE067-PAB
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            Price (USD)
            640.00
            1 mg
             
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            Cite Glyceraldehyde-3-phosphate dehydrogenase Polyclonal Antibody

            Product Details

            NE067-PAB

            Applications
            Tested Dilution
            Publications

            Immunoprecipitation (IP)

            Assay-dependent
            -
            Product Specifications

            Species Reactivity

            Yeast

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Saccharomyces cerevisiae Glyceraldehyde-3-phosphate dehydrogenase

            Conjugate

            Unconjugated

            Form

            Lyophilized

            Concentration

            2 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with 0.5% dextran

            Contains

            no preservative

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

            Cross-reactivities against enzymes of other sources may occur but have not been determined.

            Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

            Target Information

            GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Bioinformatics

            Protein Aliases: EC 1.2.1.12; HGNC:4141; MGC88685

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            It has to be done as per old AB suggested Products section.
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