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Proteintech

Glycerokinase Polyclonal Antibody

8 References

View all (25) Glycerol kinase antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)

Application

Glycerokinase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

Glycerokinase Antibody (13360-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 13360-1-AP (Glycerokinase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Glycerokinase Antibody in Western Blot (WB)

Glycerokinase Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13360-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glycerokinase Fusion Protein Ag4175 (1-357 aa encoded by BC037549)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAASKKAVL GPLVGAVDQG TSSTRFLVFN SKTAELLSHH QVEIKQEFPR EGWVEQDPKE ILHSVYECIE KTCEKLGQLN IDISNIKAIG VSNQRETTVV WDKITGEPLY NAVVWLDLRT QSTVESLSKR IPGNNNFVKS KTGLPLSTYF SAVKLRWLLD NVRKVQKAVE EKRALFGTID SWLIWSLTGG VNGGVHCTDV TNASRTMLFN IHSLEWDKQL CEFFGIPMEI LPNVRSSSEI YGLMKAGALE GVPISGCLGD QSAALVGQMC FQIGQAKNTY GTGCFLLCNT GHKCVFSDHG LLTTVAYKLG RDKPVYYALE GSVAIAGAVI RWLRDNLGII KTSEEIEKLA KEVGTSYG (1-357 aa encoded by BC037549)

Target Information

The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.

Molecular metabolism

Ford BE,Chachra SS,Rodgers K,Moonira T,Al-Oanzi ZH,Anstee QM,Reeves HL,Schattenberg JM,Fairclough RJ,Smith DM,Tiniakos D,Agius L

Thu Jun 01 00:00:00 UTC 2023

The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.

Molecular metabolism

Ford BE,Chachra SS,Rodgers K,Moonira T,Al-Oanzi ZH,Anstee QM,Reeves HL,Schattenberg JM,Fairclough RJ,Smith DM,Tiniakos D,Agius L

Thu Jun 01 00:00:00 UTC 2023

The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.

Molecular metabolism

Ford BE,Chachra SS,Rodgers K,Moonira T,Al-Oanzi ZH,Anstee QM,Reeves HL,Schattenberg JM,Fairclough RJ,Smith DM,Tiniakos D,Agius L

Thu Jun 01 00:00:00 UTC 2023

The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.

Molecular metabolism

Ford BE,Chachra SS,Rodgers K,Moonira T,Al-Oanzi ZH,Anstee QM,Reeves HL,Schattenberg JM,Fairclough RJ,Smith DM,Tiniakos D,Agius L

Thu Jun 01 00:00:00 UTC 2023

Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice.

Arteriosclerosis, thrombosis, and vascular biology

Ma C,Wang X,Zhang L,Zhu X,Bai J,He S,Mei J,Jiang J,Guan X,Zheng X,Qu L,Zhu D

Sat Jul 01 00:00:00 UTC 2023

Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice.

Arteriosclerosis, thrombosis, and vascular biology

Ma C,Wang X,Zhang L,Zhu X,Bai J,He S,Mei J,Jiang J,Guan X,Zheng X,Qu L,Zhu D

Sat Jul 01 00:00:00 UTC 2023

Serine palmitoyltransferase assembles at ER-mitochondria contact sites.

Life science alliance

Aaltonen MJ,Alecu I,König T,Bennett SA,Shoubridge EA

Tue Feb 01 00:00:00 UTC 2022

O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.

Journal of advanced research

Zhang H,Qi J,Pei J,Zhang M,Shang Y,Li Z,Wang Y,Guo J,Sun K,Fan J,Sui L,Xu Y,Kong L,Kong Y

Tue Mar 01 00:00:00 UTC 2022

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

Frontiers in cell and developmental biology

Xu Y,Han Q,Ma C,Wang Y,Zhang P,Li C,Cheng X,Xu H

Tue Aug 03 00:00:00 UTC 2021

Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice.

Journal of applied toxicology : JAT

Zeng N,Jiang H,Fan Q,Wang T,Rong W,Li G,Li R,Xu D,Guo T,Wang F,Zeng L,Huang M,Zheng J,Lu F,Chen W,Hu Q,Huang Z,Wang Q

Thu Feb 01 00:00:00 UTC 2018

Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice.

Journal of applied toxicology : JAT

Zeng N,Jiang H,Fan Q,Wang T,Rong W,Li G,Li R,Xu D,Guo T,Wang F,Zeng L,Huang M,Zheng J,Lu F,Chen W,Hu Q,Huang Z,Wang Q

Thu Feb 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: ASTP; ATP-stimulated glucocorticoid-receptor translocation promoter; ATP-stimulated glucocorticoid-receptor translocaton promoter; ATP:glycerol 3-phosphotransferase; EC 2.7.1.30; GK 3; GK3P; GKl; GKP3; GKTB; Glycerokinase; Glycerokinase 3; Glycerol kinase; Glycerolkinase

Gene Aliases: ASTP; D930012N15Rik; GK; GK1; GKD; Gyk

UniProt ID: (Human) P32189, (Rat) Q63060, (Mouse) Q64516

Entrez Gene ID: (Human) 2710, (Rat) 79223, (Mouse) 14933

Function(s)

glycerol kinase activity protein binding ATP binding histone binding nucleotide binding kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor carbohydrate kinase

Process(es)

glycerol metabolic process triglyceride metabolic process response to cold phosphorylation regulation of fatty acid metabolic process triglyceride biosynthetic process glycerol catabolic process response to drug glucose homeostasis response to ethanol glycerol-3-phosphate biosynthetic process glycerol-3-phosphate metabolic process response to organic substance carbohydrate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Glycerokinase Polyclonal Antibody

Glycerokinase Antibody (13360-1-AP) in ICC/IF

Product Details

13360-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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