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Invitrogen
Glypican 3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Glypican 3 Antibody (PA5-47256) in ICC/IF
Immunocytochemistry analysis of Glypican 3 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in Glypican 3 polyclonal antibody (Product # PA5-47256) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cyto... View More
Product Details
PA5-47256
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 3 Gln25-Val558
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608607
Product Specific Information
In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Glypican 3 is observed and less than 5% cross-reactivity with recombinant human (rh) Glypican 2, rhGlypican 5, and rhGlypican 6 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glypican proteoglycan 3; Glypican-3; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; secreted glypican-3
Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1
UniProt ID: (Human) P51654
Entrez Gene ID: (Human) 2719
retinoid metabolic process
branching involved in ureteric bud morphogenesis
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
anatomical structure morphogenesis
anterior/posterior axis specification
body morphogenesis
negative regulation of peptidase activity
glycosaminoglycan metabolic process
bone mineralization
osteoclast differentiation
lung development
positive regulation of BMP signaling pathway
embryonic hindlimb morphogenesis
positive regulation of protein catabolic process
positive regulation of endocytosis
negative regulation of smoothened signaling pathway
positive regulation of smoothened signaling pathway
negative regulation of growth
positive regulation of glucose import
negative regulation of epithelial cell proliferation
coronary vasculature development
mesenchymal cell proliferation involved in ureteric bud development
mesonephric duct morphogenesis
cell proliferation involved in metanephros development
negative regulation of canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway, planar cell polarity pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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