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Invitrogen
Goat anti-Bovine IgG (H+L) Secondary Antibody, HRP
Application
FIGURE: 1 / 1
Bovine IgG (H+L) Secondary Antibody (A18751) in WB
Western blot was performed using Goat anti-Bovine IgG (H+L) Secondary Antibody, HRP (Product # A18751) and ~55 kDa bandcorresponding to Bovine IgG Heavy Chain was observed in Bovine IgG. Purified protein (100 ng) of Canine IgG (Lane 1), Feline IgG (Lane 2), Bovine IgG (Lane 3), Rabbit IgG (Lane 4), Goat IgG (Lane 5), Rat IgG (Lane 6), Mouse IgG(Lane 7), Sheep IgG (Lane 8), Human IgG (Lane 9) wereelectrophor... View More
Product Details
A18751
Applications
Tested Dilution
Western Blot (WB)
1:500-1:20,000
Immunohistochemistry (IHC)
1:500-1:20,000
ELISA (ELISA)
1:500-1:20,000
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Bovine IgG (H+L)
Conjugate
HRP
Form
Lyophilized
Concentration
2.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% ProClin 150
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2535528
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin bovine serum immunoglobulins.
Target Information
Anti-Bovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for bovine (cow, ox) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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