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Application
FIGURE: 1 / 9
Human IgG (H+L) Secondary Antibody (A56021) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized HEK293E cells that overexpress SARS-CoV-2 Spike Protein (RBD) Recombinant Human Monoclonal Antibody (P06DHu) (Product # 703973, Lambda Light chain containing mAb) and detected using Goat anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A56021, 1:1,000). Panel a) shows representative image ... View More
Product Details
A56021
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:500 - 1:1,000
Flow Cytometry (Flow)
1:1,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Human IgG
Conjugate
Alexa Fluor™ Plus 488
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.5
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
AB_2937233
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
This Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody specifically detects human IgG Heavy and the Light chain with no cross-reactivity to other species immunoglobulins except moderate cross-reactivity towards Monkey IgG.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
Superclonal Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Immunocytochemistry
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