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Invitrogen
Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody, HRP
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Human IgG1 (Heavy chain) Secondary Antibody (A55739) in WB
Western blot analysis was performed on whole-cell extracts (30 µg lysate) of T-47D (Lane 1), DU 145 (Lane 2), HCT 116 (Lane 3) and IMR-32 (Lane 4). The blot was probed with Mucin 1/Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, 1:1,000 dilution) and detected by Goat anti-Human IgG1 (Heavy Chain) Recombinant Monoclonal Antibody, HRP (Product # A55739, 1:1,000 dilution). Chemilumine... View More
Product Details
A55739
Applications
Tested Dilution
Western Blot (WB)
1:1,000
ELISA (ELISA)
1:1,000-1:2,000
Peptide Array (Array)
1:1,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
3H60L9
Immunogen
Purified Human IgG
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with proprietary stabilizer
Contains
proprietary preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_2925764
Target
IgG1 (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody specifically detects human IgG1 heavy chain with no cross-reactivity to human IgG2, IgG3, IgG4, IgA or IgM or various species immunoglobulins, including monkey IgG.
The sensitivity and specificity of each lot is confirmed using ELISA.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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