Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Biotin
Promotions
View available promotion(s)
Promo Code: P5877545 Don't miss out: buy 3, only pay for 2 From June 2-13 take advantage of our 3 for 2 promotion. Whether you are restocking essentials or trying something new, now is a great time to get more for your money!. 자세히 알아보기
Promo Code: RPUZZ25 Stock up on essentials to piece your discovery together Until June 27, save up to $650 and get an exclusive lab-themed hidden-object puzzle. 자세히 알아보기
Application
FIGURE: 1 / 1
Rabbit IgG (H+L) Secondary Antibody (31820) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product # 702211, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin (Product # 31820) at dilutions 1:50,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,... View More
Product Details
31820
Applications
Tested Dilution
Western Blot (WB)
1:50,000-1:200,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
ELISA (ELISA)
1:100
In Situ Hybridization (ISH)
Assay-dependent
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Biotin
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.8, with 3mg/mL BSA
Contains
0.08% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228340
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31820 has been successfully used in Western blot, IF, ICC, ISH, and IHC applications.
Product # 31820 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody cross-reacts with rabbit IgM (25%). However, this antibody may cross-react with immunoglobulins from other species.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support