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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
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Application
FIGURE: 1 / 32
Rabbit IgG (H+L) Secondary Antibody (32460) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of K562 (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-Cyclophilin B Rabbit Polyclonal Antibody (Product # TAB1002, 0.5 µg/mL) and detected using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Product # 32460) at dilutions 1:500 (Fig. 1), 1:2,000 (Fig. 2) and 1:5,000 (Fig. 3). A 23 kDa band corresponding to Cy... View More
Product Details
32460
Applications
Tested Dilution
Western Blot (WB)
1:500-1:5,000
Immunohistochemistry (IHC)
1:6-1:60
ELISA (ELISA)
1:60-1:500
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG, whole molecule
Conjugate
HRP
Form
Liquid
Concentration
0.01 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with proprietary stabilizer
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1185567
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
This antibody may cross-react with immunoglobulins from other species.
Working dilutions for Western blotting using SuperSignal West Substrates:
SuperSignal West Pico Substrate - 1:250-1:1250
SuperSignal West Dura Substrate - 1:600-1:3000
SuperSignal West Femto Substrate - 1:1250-1:6000
ECL Western Blotting Substrate - 1:20-1:125
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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