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H3.3 G34W oncohistone mutant Antibody (712828) in WB
Western blot was performed using Anti-H3.3 G34W Recombinant Rabbit Polyclonal Antibody (Product # 712828) and a 17 kDa band corresponding to H3.3 G34W was observed in histone H3.3 G34W overexpressing HEK-293T cells confirming the specificity of H3.3 G34W antibody. Modified whole cell extracts (1% SDS) (30 µg lysate) from control (untransfected) (Lane 1), FLAG-tagged histone H3.3 G34W overexpressing HEK-293T... View More
Product Details
712828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
4HCLC
Immunogen
Peptide corresponding to human H3.3 G34W [aa30-aa39]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2866483
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H3 histone, family 3B (H3.3B); H3.3; H3.3 G34W; H3B; H3FT; H3G34W; H3t; Histone 3.3; Histone cluster 3; Histone H3.3; Histone H3.3 G34W; Histone H3.3G34W
Gene Aliases: H3-3A; H3-3B; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; PP781
UniProt ID: (Human) P84243
Entrez Gene ID: (Human) 3020, (Human) 3021
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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