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HAT Tag Antibody (MA5-36278) in WB
Western Blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 µg of HAT Tag Monoclonal Antibody (9A2F8) (Product # MA5-36278) to immunoprecipitate and 1 µg/mL anti-GGP1 antibody to detect.
Product Details
MA5-36278
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
9A2F8
Immunogen
HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890554
Target Information
HAT Tag (Histidine Affinity Tag) is a peptide epitope (KDHLIHNVHKEFHAHAHNK) used in protein expression and purification. In comparison to the His tag, it is rich in histidines and has high affinity for immobilized metal ions such as cobalt, nickel, and zinc. However, the sequence is naturally-occurring, featuring non-adjacent His residues that result in a lower overall charge than tags with consecutive histidines. HAT tag antibodies are available for the detection of tagged fusion proteins is meant to resemble the solubility of wild-type proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hat; HAT tag; Hat-tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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