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FIGURE: 1 / 1
HCoV-NL63 Spike S2 Antibody (PA5-120766) in WB
Western blot analysis of HCoV-NL63 Spike S2 in extracts of Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag). Samples were incubated with HCoV-NL63 Spike S2 Polyclonal antibody (Product # PA5-120766) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120766
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of coronavirus Spike S2 (YP_0037671)
Conjugate
Form
Liquid
Concentration
2.49 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914338
Product Specific Information
Positive test controls include: Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag).
Immunogen sequence: VNISRVELHT VIPDYVDVNK TLQEFAQNLP KYVKPNFDLT PFNLTYLNLS SELKQLEAKT ASLFQTTVEL QGLIDQINST YVDLKLLNRF ENYIKWPWWV W
Target Information
S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; HCoV; NL63; Spike
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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