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Invitrogen
HDAC3 Monoclonal Antibody (B2-G9)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
HDAC3 Antibody (MA5-42578) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of Histone Deacetylase 3(HDAC3) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HDAC3 Monoclonal Antibody (Product # MA5-42578) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is D... View More
Product Details
MA5-42578
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
B2-G9
Immunogen
Synthetic peptide within C-terminal human HDAC3
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911719
Product Specific Information
Positive Control: MCF-7 lysate, PC-12 cell lysate, MCF-7, human kidney tissue, mouse kidney tissue, Hela.
Subcellular Location: Cytosol, Nucleus, Cytoplasm.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45
Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2
UniProt ID: (Human) O15379, (Mouse) O88895, (Rat) Q6P6W3
Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
transcription factor binding
enzyme binding
cyclin binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
NF-kappaB binding
DNA binding
hydrolase activity
histone deacetylase activity (H3-K14 specific)
chromatin DNA binding
histone deacetylase activity (H3-K9 specific)
histone deacetylase activity (H4-K16 specific)
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
GTPase binding
NAD-dependent histone deacetylase activity (H3-K18 specific)
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
protein deacetylation
regulation of mitotic cell cycle
chromatin modification
histone deacetylation
regulation of protein stability
positive regulation of TOR signaling
negative regulation of interleukin-1 production
negative regulation of tumor necrosis factor production
circadian regulation of gene expression
regulation of multicellular organism growth
positive regulation of transcription factor import into nucleus
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
negative regulation of protein export from nucleus
spindle assembly
cellular response to fluid shear stress
positive regulation of type B pancreatic cell apoptotic process
negative regulation of cardiac muscle cell differentiation
negative regulation of myotube differentiation
response to nutrient levels
response to drug
histone H3-K9 modification
histone H3 deacetylation
histone H4 deacetylation
cellular response to mechanical stimulus
cellular response to parathyroid hormone stimulus
response to dexamethasone
circadian rhythm
negative regulation of apoptotic process
cellular lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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