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Invitrogen
HDAC8 Monoclonal Antibody (HDAC8-48)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
HDAC8 Antibody (MA1-25458) in WB
Western blot was performed using Anti-HDAC8 Monoclonal Antibody (HDAC8-48) (Product # MA1-25458) and a 41 kDa band corresponding to HDAC8 was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of PC-3 (Lane 1), SH-SY5Y (Lane 2), K-562 (Lane 3), A549 (Lane 4), HeLa (Lane 5), HEK-293 (Lane 6), Jurkat (Lane 7) and Raji (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iB... View More
Product Details
MA1-25458
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HDAC8-48
Immunogen
Recombinant, full length human protein.
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_794433
Product Specific Information
Recommended positive controls: HeLa nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD8; Histone deacetylase 8; histone deacetylase-like 1; Protein deacetylase HDAC8; Protein decrotonylase HDAC8
Gene Aliases: CDA07; CDLS5; HD8; HDAC8; HDACL1; MRXS6; RPD3; WTS
UniProt ID: (Human) Q9BY41
Entrez Gene ID: (Human) 55869
negative regulation of transcription from RNA polymerase II promoter
chromatin assembly or disassembly
transcription, DNA-templated
sister chromatid cohesion
chromatin modification
negative regulation of protein ubiquitination
regulation of protein stability
regulation of telomere maintenance
histone H3 deacetylation
regulation of cohesin loading
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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