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FIGURE: 1 / 2
HDAC8 Antibody (CF809651) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC8 (RC208390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC8. Positive lysates LY413013 (100 µg) and LC413013 (20 µg) can be purchased separately from OriGene.
Product Details
CF809651
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1A4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-243 of human HDAC8 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD8; Histone deacetylase 8; histone deacetylase-like 1; Protein deacetylase HDAC8; Protein decrotonylase HDAC8
Gene Aliases: 2610007D20Rik; CDA07; CDLS5; HD8; HDAC8; HDACL1; MRXS6; RGD1562895; RPD3; WTS
UniProt ID: (Human) Q9BY41, (Rat) B1WC68, (Mouse) Q8VH37
Entrez Gene ID: (Human) 55869, (Rat) 363481, (Mouse) 70315
histone deacetylase activity
protein binding
transcription factor binding
Hsp70 protein binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
metal ion binding
Hsp90 protein binding
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
NAD-dependent histone deacetylase activity (H3-K18 specific)
hydrolase activity
histone deacetylase activity (H3-K14 specific)
histone deacetylase activity (H3-K9 specific)
histone deacetylase activity (H4-K16 specific)
transcription, DNA-templated
regulation of transcription, DNA-templated
sister chromatid cohesion
histone H3-K9 modification
histone H3 deacetylation
histone H4 deacetylation
regulation of cohesin loading
chromatin modification
histone deacetylation
negative regulation of transcription from RNA polymerase II promoter
chromatin assembly or disassembly
negative regulation of protein ubiquitination
regulation of protein stability
regulation of telomere maintenance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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