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FIGURE: 1 / 1
HDAC9 Antibody (CL488-67364) in Flow
1X10^6 HeLa cells were intracellularly stained with 0.4 µg CoraLite® Plus 488 Anti-Human HDAC9 (CL488-67364, Clone:1G6C5) (red), or 0.4 µg Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Product Details
CL488-67364
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1G6C5
Immunogen
Recombinant protein
Conjugate
Excitation/Emission Max
493/522 nm
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol, 0.5% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: PAVPSQLNAS NSLKEKQKCE TQTLRQGVPL PGQYGGSIPA SSSHPHVTLE GKPPNSSHQA LLQHLLLKEQ MRQQKLLVAG GVPLHPQSPL ATKERISPGI RGTHKL
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp779K1053; HD7; HD9; hdac-9; histone deacetylase 4/5-related protein; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR
Gene Aliases: HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR
UniProt ID: (Human) Q9UKV0
Entrez Gene ID: (Human) 9734
transcription corepressor activity
histone deacetylase activity
protein kinase C binding
protein binding
transcription factor binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
metal ion binding
repressing transcription factor binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
response to amphetamine
transcription, DNA-templated
inflammatory response
heart development
chromatin modification
histone deacetylation
neuron differentiation
B cell differentiation
cellular response to insulin stimulus
peptidyl-lysine deacetylation
B cell activation
negative regulation of transcription, DNA-templated
regulation of skeletal muscle fiber development
regulation of striated muscle cell differentiation
histone H3 deacetylation
histone H4 deacetylation
positive regulation of cell migration involved in sprouting angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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