Proteintech

HECTD3 Polyclonal Antibody

FIGURE: 1 / 3

HECTD3 Antibody (11487-1-AP) in WB

MCF-7 cells were subjected to SDS PAGE followed by western blot with 11487-1-AP (HECTD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HECTD3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11487-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HECTD3 Fusion Protein Ag1998 (3-213 aa encoded by BC019105)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VMEGMDKET FEFKFGKELT FTTVLSDQQV VELIPGGAGI VVGYGDRSRF IQLVQKARLE ESKEQVAAMQ AGLLKVVPQA VLDLLTWQEL EKKVCGDPEV TVDALRKLTR FEDFEPSDSR VQYFWEALNN FTNEDRSRFL RFVTGRSRLP ARIYIYPDKL GYETTDALPE SSTCSSTLFL PHYASAKVCE EKLRYAAYNC VAIDTDMSPW EE (3-213 aa encoded by BC019105)

Target Information

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells.

Cell death & disease

Li J,Sun Y,Zhao X,Ma Y,Xie Y,Liu S,Hui B,Shi X,Sun X,Zhang X

Sat Apr 08 00:00:00 EDT 2023

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway.

British journal of cancer

Zhang G,Tan R,Wan S,Yang R,Hu X,Zhao E,Ding X,Zhang J,Li B,Liang P,Cui H

Tue Nov 01 00:00:00 EDT 2022

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway.

British journal of cancer

Zhang G,Tan R,Wan S,Yang R,Hu X,Zhao E,Ding X,Zhang J,Li B,Liang P,Cui H

Tue Nov 01 00:00:00 EDT 2022

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway.

British journal of cancer

Zhang G,Tan R,Wan S,Yang R,Hu X,Zhao E,Ding X,Zhang J,Li B,Liang P,Cui H

Tue Nov 01 00:00:00 EDT 2022

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway.

British journal of cancer

Zhang G,Tan R,Wan S,Yang R,Hu X,Zhao E,Ding X,Zhang J,Li B,Liang P,Cui H

Tue Nov 01 00:00:00 EDT 2022

HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC.

Cell death discovery

Zhang G,Zhu Q,Yan X,Ci M,Zhao E,Hou J,Wan S,Lü M,Cui H

Sat Apr 09 00:00:00 EDT 2022

E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3.

Nature communications

Gao P,Ma X,Yuan M,Yi Y,Liu G,Wen M,Jiang W,Ji R,Zhu L,Tang Z,Yu Q,Xu J,Yang R,Xia S,Yang M,Pan J,Yuan H,An H

Fri Feb 19 00:00:00 EST 2021

uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.

Frontiers in oncology

Zheng Z,Hong D,Zhang X,Chang Y,Sun N,Lin Z,Li H,Huang S,Zhang R,Xie Q,Huang H,Jin H

Tue Jun 08 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase HECTD3; HECT domain containing E3 ubiquitin protein ligase 3; HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3; probable E3 ubiquitin-protein ligase HECTD3

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Gene Aliases: 1700064K09Rik; AI467540; AW743312; HECTD3

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UniProt ID: (Human) Q5T447, (Mouse) Q3U487

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Entrez Gene ID: (Human) 79654, (Mouse) 76608

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Function(s)

ubiquitin-protein transferase activity protein binding ligase activity syntaxin binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

Process(es)

protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process protein ubiquitination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HECTD3 Polyclonal Antibody

HECTD3 Antibody (11487-1-AP) in WB

Product Details

11487-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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