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Application
FIGURE: 1 / 1
HERC4 Antibody (MA5-55646) in WB
Western blot analysis of HERC4 in 15 µg of rat liver tissue. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. HERC4 band was visualized using ECL Western Blotting Substrate. Incubation with primary HERC4 monoclonal antibody (Product # MA5-55646) at a dilution of 1 µg/mL was used.
Product Details
MA5-55646
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K29058_14H6
Immunogen
Recombinant human HERC4 (immunogen range is 21-260).
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_3667878
Product Specific Information
Sequence of this protein is as follows: IVLEPRKSDF FINKRVRDVG CGLRHTVFVL DDGTVYTCGC NDLGQLGHEK SRKKPEQVVA LDAQNIVAVS CGEAHTLALN DKGQVYAWGL DSDGQLGLVG SEECIRVPRN IKSLSDIQIV QVACGYYHSL ALSKASEVFC WGQNKYGQLG LGTDCKKQTS PQLLKSLLGI PFMQVAAGGA HSFVLTLSGA IFGWGRNKFG QLGLNDENDR YVPNLLKSLR SQKIVYICCG EDHTAALTKE
Target Information
Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HECT domain and RCC1-like domain-containing protein 4; hect domain and RLD 4; HECT-type E3 ubiquitin transferase HERC4; Probable E3 ubiquitin-protein ligase HERC4
Gene Aliases: HERC4; KIAA1593
UniProt ID: (Human) Q5GLZ8, (Rat) Q5PQN1
Entrez Gene ID: (Human) 26091, (Rat) 309758
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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