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Invitrogen
HES5 Recombinant Rabbit Monoclonal Antibody (4S4U7)
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Application
FIGURE: 1 / 3
HES5 Antibody (MA5-38214) in IHC (P)
Immunohistochemistry analysis of HES5 in paraffin-embedded human lung cancer. Samples were incubated with HES5 Monoclonal antibody (Product # MA5-38214) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-38214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4S4U7
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 12-142 of human HES5 (Q5TA89)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898131
Product Specific Information
Positive Samples: Mouse brain, Mouse spleen, Rat brain, Rat spleen
Immunogen sequence: SPKEKNRLRK PVVEKMRRDR INSSIEQLKL LLEQEFARHQ PNSKLEKADI LEMAVSYLKH SKAFVAAAGP KSLHQDYSEG YSWCLQEAVQ FLTLHAASDT QMKLLYHFQR PPAAPAAPAK EPKAPGAAPP P
Target Information
Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 is reported to be a down stream effector of the Notch Signaling Pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma. Reports also suggest that HES5 is abundantly expressed in osteoarthritis along with Notch1 and Jagged1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb38; Class B basic helix-loop-helix protein 38; Hairy and enhancer of split 5; RP3-395M20.5; Transcription factor HES-5
Gene Aliases: BHLHB38; Hes-5; HES5
UniProt ID: (Human) Q5TA89, (Rat) Q03062, (Mouse) P70120
Entrez Gene ID: (Human) 388585, (Rat) 79225, (Mouse) 15208
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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