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FIGURE: 1 / 1
HFE Antibody (MA5-44881) in WB
Western blot analysis of HFE in different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in HFE Monoclonal antibody (Product # MA5-44881) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate; Lane 2: A431 cell lysate.
Product Details
MA5-44881
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE58-66
Immunogen
Synthetic peptide within human HFE aa 299-348/348.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931337
Target Information
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin. It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hereditary hemochromatosis protein; hereditary hemochromatosis protein HLA-H; HFE 1; high Fe; HLA H; HLA-H; MGC10379; MGC103790; MGC:150812; MHC class I-like protein HFE
Gene Aliases: HFE; HFE1; HH; HLA-H; HLAH; MVCD7; TFQTL2
UniProt ID: (Human) Q30201
Entrez Gene ID: (Human) 3077
antigen processing and presentation of peptide antigen via MHC class I
negative regulation of T cell antigen processing and presentation
negative regulation of T cell cytokine production
protein complex assembly
cellular iron ion homeostasis
acute-phase response
immune response
female pregnancy
response to iron ion
cellular response to iron ion starvation
positive regulation of gene expression
positive regulation of pathway-restricted SMAD protein phosphorylation
antigen processing and presentation
BMP signaling pathway
positive regulation of protein binding
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
hormone biosynthetic process
positive regulation of receptor-mediated endocytosis
iron ion homeostasis
multicellular organismal iron ion homeostasis
cellular response to iron ion
positive regulation of peptide hormone secretion
liver regeneration
iron ion import into cell
negative regulation of receptor binding
positive regulation of receptor binding
positive regulation of ferrous iron import into cell
negative regulation of antigen processing and presentation of endogenous peptide antigen via MHC class I
positive regulation of ferrous iron binding
positive regulation of transferrin receptor binding
response to iron ion starvation
regulation of protein localization to cell surface
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of receptor activity
positive regulation of receptor activity
negative regulation of CD8-positive, alpha-beta T cell activation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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