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HGK Antibody (PA5-30579) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HGK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HGK Polyclonal Antibody (Product # PA5-30579) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-30579
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 383 and 437 of Human MAP4K4
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548053
Product Specific Information
Recommended positive controls: 293T, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (100%), Rat (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MAP4K4 (HGK) is a serine/threonine kinase which plays an important role in stress-activated MAPK core signaling pathways, the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 31; FLJ10410; FLJ20373; FLJ90111; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck-interacting kinase; Ste20 group protein kinase HGK
Gene Aliases: FLH21957; HEL-S-31; HGK; KIAA0687; MAP4K4; MEKKK4; NIK
UniProt ID: (Human) O95819
Entrez Gene ID: (Human) 9448
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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