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Invitrogen

HICE1 Polyclonal Antibody

3 References

View all (11) HICE1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

Application

HICE1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

HICE1 Antibody (PA5-21331) in ICC/IF

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1 (Product # PA5-21331) antibody at 1:50-1:200 dilution.

HICE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HICE1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA5-21331

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to human HICE1.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153508

Product Specific Information

Recommended positive controls: K562, HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Hice1 contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment.

Cell reports

Risteski P,Božan D,Jagrić M,Bosilj A,Pavin N,Tolić IM

PA5-21331 was used in Immunocytochemistry-immunoflourescence to introduce a flux-driven centering model that relies on flux generated by forces within the overlaps of bridging and kinetochore fibers.

Tue Aug 02 00:00:00 UTC 2022

Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers.

eLife

Štimac V,Koprivec I,Manenica M,Simunić J,Tolić IM

PA5-21331 was used in Immunocytochemistry and Western Blotting to analyze the previously indistinguishable microtubule arrangements within the crowded metaphase plate area and demonstrate that augmin is vital for the formation of uniformly arranged parallel units consisting of sister kinetochore fibers connected by a bridging fiber.

Fri Oct 21 00:00:00 UTC 2022

Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers.

eLife

Štimac V,Koprivec I,Manenica M,Simunić J,Tolić IM

Fri Oct 21 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: HAUS augmin-like complex subunit 8; HAUS augmin-like complex, subunit 8; Hec1-interacting and centrosome-associated 1; HEC1/NDC80 interacting, centrosome associated 1; HEC1/NDC80-interacting centrosome-associated protein 1; MGC20533; NY SAR 48; NYSAR48; Sarcoma antigen NY-SAR-48

Gene Aliases: DGT4; HAUS8; HICE1; NY-SAR-48

UniProt ID: (Human) B4DJA7

Entrez Gene ID: (Human) 93323

Function(s)

Process(es)

G2/M transition of mitotic cell cycle mitotic nuclear division spindle assembly centrosome organization cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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