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Product Details
BS-15487R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human HIF Prolyl Hydroxylases, amino acids 201-300.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20262; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; PH 4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; Transmembrane prolyl 4-hydroxylase
Gene Aliases: 4933406E20Rik; AI853847; BB128974; EGLN4; HIFPH4; P4H-TM; P4HTM; PH-4; PH4; PHD4; RGD1311848
UniProt ID: (Human) Q9NXG6, (Mouse) Q8BG58
Entrez Gene ID: (Human) 54681, (Mouse) 74443, (Rat) 301008
iron ion binding
calcium ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
L-ascorbic acid binding
sulfonate dioxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
protein modifying enzyme
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