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Application
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HIPK3 Antibody (PA5-99799) in ICC/IF
Immunocytochemistry analysis of HIPK3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HIPK3 polyclonal antibody (Product # PA5-99799) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99799
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HIPK3(Accession Q9H422), corresponding to amino acid residues P930-V980.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818732
Product Specific Information
Antibody detects endogenous levels of total HIPK3.
Target Information
HIPK3 is a serine/threonine kinase involved in signal transduction, cellular communication, and multidrug resistance of tumors and plays a role in the phosphorylation of FADD. HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Androgen receptor-interacting nuclear protein kinase; ANPK; Fas-interacting serine/threonine-protein kinase; FIST; Homeodomain-interacting protein kinase 3; Homolog of protein kinase YAK1; RP1-8L15.1
Gene Aliases: DYRK6; FIST3; HIPK3; PKY; YAK1
UniProt ID: (Human) Q9H422
Entrez Gene ID: (Human) 10114
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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