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Invitrogen
HIV1 p24 Polyclonal Antibody
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Application
FIGURE: 1 / 1
HIV1 p24 Antibody (PA1-4951) in WB
Detection of HIV-1 p24 and precursor proteins p55 and p41 by Western blotting using an HIV1 p24 Polyclonal Antibody (Product # PA1-4951). Lane 1: Extract of MT4 cells. Lane2: Extract of MT4 cells infected with HIV-1(LAI strain). The antiserum was diluted 1:2,500. The upper bands are precursors of p55 of p24 and the processed intermediate proteins.
Product Details
PA1-4951
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified full-size recombinant HIV1 p24, subtype B expressed in E. coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_612271
Product Specific Information
PA1-4951 detects the HIV-1 p24 protein in HIV samples.
PA1-4951 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~24 kDa protein, a ~55 kDa protein, and a ~41 kDa protein, corresponding respectively to HIV-p24 and to its precursors p55 and p41, in HIV samples.
PA1-4951 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of the HIV-1 virus.
Recombinant HIV-1 Gag p24 protein (Cat. # RP-4916 or RP-4917) can be purchased for control experiments in Western blot assays.
Target Information
HIV-1 Gag p24 is a capsid protein that constitutes the core of the AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. The HIV-1 Gag p24 protein is indispensable to the reproduction of AIDS virus, and constitutes an essential element for the AIDS virus particle construction. HIV-1 Gag p24 is detectable from the early stage of AIDS virus infection, and is used as a marker for observation of the development in the patient’s condition after treatment, as it indicated the amount of virus in the blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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