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Application
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HK1 Antibody (702742) in WB
Western blot analysis was performed on Whole cell extracts (60 µg lysate) of PC-3 (Lane 1), K-562 (Lane 2), U-87 MG (Lane 3), MCF-7 (Lane 4), A-431 (Lane 5) PANC-1 (Lane 6) and A549 (Lane 7). The blots were probed with Anti-Hexokinase 1 Recombinant Rabbit Monoclonal Antibody (Product # 702742, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to Hexokinase 1 was observed across the... View More
Product Details
702742
Product Specifications
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
18H16L7
Immunogen
Peptides corresponding to human HK1 [1) aa315-aa334 2) aa719-aa738]
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2724452
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Dog, Bovine
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the ARHGAP family of Rho/Rac/Cdc42-like GTPase activating proteins. The encoded protein interacts with the Ras-related protein Gem through its N-terminal domain. Separately, it interacts with RhoA through a RhoGAP domain, and stimulates RhoA-dependent GTPase activity. Alternatively spliced variants that encode different protein isoforms have been described but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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