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Application
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HLA-DR1 Antibody (MA1-10354) in Flow
Separation of human HLA-DR1 (empty) positive HLA-DR positive cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE Monoclonal antibody (Product # MA1-10354) (concentration in sample 9 µg/mL).
Product Details
MA1-10354
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
MEM-267
Immunogen
Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152797
Product Specific Information
This antibody binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Target Information
HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DW2.2/DR2.2; FLJ75017; FLJ76359; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA-DRB1; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class II antigen; MHC class II antigen DRA; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; XXbac-BPG161M6.1
Gene Aliases: DRB1; DRw10; HLA-DR1B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; MLRW; SS1
UniProt ID: (Human) P01903, (Human) Q4PRC3
Entrez Gene ID: (Human) 3122, (Human) 3123
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