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Invitrogen
HMBOX1 Polyclonal Antibody
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Application
FIGURE: 1 / 3
HMBOX1 Antibody (PA5-115366) in ICC/IF
Immunocytochemistry analysis of HMBOX1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115366). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HMBOX1(Accession Q6NT76), corresponding to amino acid residues E42-T92.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900002
Product Specific Information
Antibody detects endogenous levels of total HMBOX1.
Target Information
HMBOX1 is located at the boundary of 8p12.3 and 8p21.1. HMBOX1 proteins are highly conserved in human, mouse, rat, chicken and Xenopus laevis. Functional HMBOX1::EGFP (enhanced green fluorescent protein) fusion protein revealed that HMBOX1 accumulated more in cytoplasm than in nucleus. HMBOX1 is a transcription repressor. Human HMBOX1 is widely expressed in 18 tissues, and it is highly expressed in pancreas. Hmbox1 is widely expressed in mouse pancreas and the expression of this gene can also be detected in pallium, hippocampus and hypothalamus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox containing 1; Homeobox telomere-binding protein 1; Homeobox-containing protein 1; homeobox-containing protein PBHNF; Telomere-associated homeobox-containing protein 1
Gene Aliases: AI451877; AI604847; F830020C16Rik; HMBOX1; HNF1LA; HOT1; PBHNF; RGD1306787; TAH1
UniProt ID: (Human) Q6NT76, (Mouse) Q8BJA3
Entrez Gene ID: (Human) 79618, (Rat) 305968, (Mouse) 219150
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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