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Proteintech

HMGCL Polyclonal Antibody

View all (12) HMGCL antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

Application

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

HMGCL Antibody (16898-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCL Antibody in Western Blot (WB)

HMGCL Antibody in Western Blot (WB)

HMGCL Antibody in Western Blot (WB)

HMGCL Antibody in Western Blot (WB)

HMGCL Antibody in Immunoprecipitation (IP)

Product Image Gallery

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

HMGCL Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 16898-1-AP) HMGCL antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

HMGCL Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HMGCL Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HMGCL Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HMGCL Antibody in Immunoprecipitation (IP) — IP result of anti-HMGCL (IP:16898-1-AP, 4ug; Detection:16898-1-AP 1:500) with mouse spleen tissue lysate 4000 ug.

Product Details

16898-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HMGCL Fusion Protein Ag10426 (1-325 aa encoded by BC010570)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAAMRKALP RRLVGLASLR AVSTSSMGTL PKRVKIVEVG PRDGLQNEKN IVSTPVKIKL IDMLSEAGLS VIETTSFVSP KWVPQMGDHT EVLKGIQKFP GINYPVLTPN LKGFEAAVAA GAKEVVIFGA ASELFTKKNI NCSIEESFQR FDAILKAAQS ANISVRGYVS CALGCPYEGK ISPAKVAEVT KKFYSMGCYE ISLGDTIGVG TPGIMKDMLS AVMQEVPLAA LAVHCHDTYG QALANTLMAL QMGVSVVDSS VAGLGGCPYA QGASGNLATE DLVYMLEGLG IHTGVNLQKL LEAGNFICQA LNRKTSSKVA QATCKL (1-325 aa encoded by B C010570)

Target Information

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation.

The Journal of biological chemistry

Goldberg EL,Letian A,Dlugos T,Leveau C,Dixit VD

Wed Mar 01 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 UTC 2023

HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility.

Hepatology international

Cui X,Yun X,Sun M,Li R,Lyu X,Lao Y,Qin X,Yu W

Sat Apr 01 00:00:00 UTC 2023

HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility.

Hepatology international

Cui X,Yun X,Sun M,Li R,Lyu X,Lao Y,Qin X,Yu W

Sat Apr 01 00:00:00 UTC 2023

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Nature cell biology

Li JT,Yin M,Wang D,Wang J,Lei MZ,Zhang Y,Liu Y,Zhang L,Zou SW,Hu LP,Zhang ZG,Wang YP,Wen WY,Lu HJ,Chen ZJ,Su D,Lei QY

Sat Feb 01 00:00:00 UTC 2020

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Cell research

Huang D,Li T,Wang L,Zhang L,Yan R,Li K,Xing S,Wu G,Hu L,Jia W,Lin SC,Dang CV,Song L,Gao P,Zhang H

Sat Oct 01 00:00:00 UTC 2016

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Cell research

Huang D,Li T,Wang L,Zhang L,Yan R,Li K,Xing S,Wu G,Hu L,Jia W,Lin SC,Dang CV,Song L,Gao P,Zhang H

Sat Oct 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxy-3-methylglutaryl-Coenzyme A lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase; HL; HMG-CoA lyase; hydroxymethylglutaricaciduria; Hydroxymethylglutaryl-CoA lyase, mitochondrial; mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase

Gene Aliases: AW476067; HL; HMGCL

UniProt ID: (Human) P35914, (Mouse) P38060, (Rat) P97519

Entrez Gene ID: (Human) 3155, (Mouse) 15356, (Rat) 79238

Function(s)

fatty-acyl-CoA binding magnesium ion binding hydroxymethylglutaryl-CoA lyase activity receptor binding manganese ion binding carboxylic acid binding protein homodimerization activity metal ion binding catalytic activity lyase activity lyase

Process(es)

liver development leucine catabolic process acyl-CoA metabolic process mitochondrion organization response to nutrient response to starvation ketone body biosynthetic process protein tetramerization response to fatty acid embryo development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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