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Invitrogen

HMGCS1 Polyclonal Antibody

4 Published Figures

5 References

View all (12) HMGCS1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (4)

Application

HMGCS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

HMGCS1 Antibody (PA5-29488) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of HMGCS1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 Polyclonal Antibody (Product # PA5-29488) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

HMGCS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCS1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HMGCS1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-29488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Horse, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546964

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.

Predicted reactivity: Mouse (97%), Rat (97%), Pig (95%), Chicken (82%), Rhesus Monkey (99%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.

Nature communications

Molina-Gonzalez I,Holloway RK,Jiwaji Z,Dando O,Kent SA,Emelianova K,Lloyd AF,Forbes LH,Mahmood A,Skripuletz T,Gudi V,Febery JA,Johnson JA,Fowler JH,Kuhlmann T,Williams A,Chandran S,Stangel M,Howden AJM,Hardingham GE,Miron VE

PA5-29488 was used in Immunohistochemistry-immunofluorescence to identify that astrocyte-oligodendrocyte interaction regulates remyelination, and reveal a drug strategy for central nervous system regeneration centred on targeting this interaction.

Thu Jun 08 00:00:00 UTC 2023

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Published figure using HMGCS1 polyclonal antibody (Product # PA5-29488) in Immunocytochemistry

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Published figure using HMGCS1 polyclonal antibody (Product # PA5-29488) in Immunocytochemistry

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Published figure using HMGCS1 polyclonal antibody (Product # PA5-29488) in Immunocytochemistry

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Published figure using HMGCS1 polyclonal antibody (Product # PA5-29488) in Immunocytochemistry

Sat May 01 00:00:00 UTC 2021

Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.

Frontiers in immunology

Menarim BC,El-Sheikh Ali H,Loux SC,Scoggin KE,Kalbfleisch TS,MacLeod JN,Dahlgren LA

PA5-29488 was used in Immunohistochemistry (Paraffin) to study differential transcriptional signatures of bone marrow mononuclear cells in response to normal and inflamed synovial fluid.

Thu Feb 10 00:00:00 UTC 2022

Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.

Frontiers in immunology

Menarim BC,El-Sheikh Ali H,Loux SC,Scoggin KE,Kalbfleisch TS,MacLeod JN,Dahlgren LA

PA5-29488 was used in Immunohistochemistry (Paraffin) to study differential transcriptional signatures of bone marrow mononuclear cells in response to normal and inflamed synovial fluid.

Thu Feb 10 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMG-CoA synthase; Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene Aliases: B130032C06Rik; HMGCS; HMGCS1

UniProt ID: (Human) Q01581, (Mouse) Q8JZK9

Entrez Gene ID: (Human) 3157, (Mouse) 208715

Function(s)

hydroxymethylglutaryl-CoA synthase activity drug binding isomerase activity protein homodimerization activity organic acid binding catalytic activity transferase activity

Process(es)

liver development lipid metabolic process cholesterol biosynthetic process brain development isoprenoid biosynthetic process male gonad development response to low light intensity stimulus response to purine-containing compound response to vitamin E response to drug response to tellurium ion response to lipoprotein particle cellular response to follicle-stimulating hormone stimulus cellular response to cholesterol steroid biosynthetic process metabolic process steroid metabolic process cholesterol metabolic process response to organonitrogen compound sterol biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMGCS1 Polyclonal Antibody

HMGCS1 Antibody (PA5-29488) in ICC/IF

Product Details

PA5-29488

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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