Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
12533-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
HNF1B Fusion Protein Ag3232 (1-310 aa encoded by BC017714)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.28 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This is a rabbit polyclonal antibody raised against the N-terminal region of human HNF1B. HNF1B exists some isoforms with MW of 61 kDa, 58 kDa, 46 kDa, and 44 kDa.
Immunogen sequence: MVSKLTSLQ QELLSALLSS GVTKEVLVQA LEELLPSPNF GVKLETLPLS PGSGAEPDTK PVFHTLTNGH AKGRLSGDEG SEDGDDYDTP PILKELQALN TEEAAEQRAE VDRMLSEDPW RAAKMIKGYM QQHNIPQREV VDVTGLNQSH LSQHLNKGTP MKTQKRAALY TWYVRKQREI LRQFNQTVQS SGNMTDKSSQ DQLLFLFPEF SQQSHGPGQS DDACSEPTNK KMRRNRFKWG PASQQILYQA YDRQKNPSKE EREALVEECN RAECLQRGVS PSKAHGLGSN LVTEVRVYNW FANRRKEEAF R (1-310 aa encoded by BC017714)
Target Information
Hepatocyte Nuclear Factor 1 (HNF1, Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor) is a member of the homeodomain-containing superfamily of transcription factors. It is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. HNF1has been shown to function in nephron development and regulates development of the embryonic pancreas. Mutations in the gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of the gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Hepatocyte nuclear factor 1-beta; hepatocyte nuclear factor-1 beta; HNF-1-beta; HNF-1B; HNF1 beta A; Homeoprotein LFB3; TCF-2; Transcription factor 2; Transcription factor 2 hepatic; Transcription factor 2 hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2, hepatic; Transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; Variant hepatic nuclear factor 1; vHNF1
Gene Aliases: FJHN; HNF-1-beta; HNF-1B; HNF-1Beta; HNF1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
UniProt ID: (Human) P35680, (Rat) P23899, (Mouse) P27889
Entrez Gene ID: (Human) 6928, (Rat) 25640, (Mouse) 21410
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter proximal region DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein complex binding
protein homodimerization activity
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
kidney development
inner cell mass cell differentiation
transcription, DNA-templated
Notch signaling pathway
response to glucose
anterior/posterior pattern specification
response to organic cyclic compound
insulin secretion
regulation of Wnt signaling pathway
hindbrain development
circadian regulation of gene expression
regulation of pronephros size
pronephric nephron tubule development
response to drug
regulation of endodermal cell fate specification
positive regulation of transcription, DNA-templated
embryonic digestive tract morphogenesis
branching morphogenesis of an epithelial tube
pronephros development
epithelial cell proliferation
positive regulation of transcription initiation from RNA polymerase II promoter
ureteric bud elongation
hepatoblast differentiation
negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis
protein-DNA complex assembly
hepatocyte differentiation
regulation of branch elongation involved in ureteric bud branching
mesonephric duct formation
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
liver development
transcription from RNA polymerase II promoter
endoderm development
response to carbohydrate
positive regulation of gene expression
endocrine pancreas development
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
embryonic morphogenesis
genitalia development
epithelium development
kidney morphogenesis
mesonephric tubule development
nephric duct development
mesonephric duct development
nephric duct formation
endoderm formation
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support