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Proteintech

HNF1B Polyclonal Antibody

26 References

View all (24) TCF2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)

Application

HNF1B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

HNF1B Antibody (12533-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HNF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

12533-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HNF1B Fusion Protein Ag3232 (1-310 aa encoded by BC017714)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against the N-terminal region of human HNF1B. HNF1B exists some isoforms with MW of 61 kDa, 58 kDa, 46 kDa, and 44 kDa.

Immunogen sequence: MVSKLTSLQ QELLSALLSS GVTKEVLVQA LEELLPSPNF GVKLETLPLS PGSGAEPDTK PVFHTLTNGH AKGRLSGDEG SEDGDDYDTP PILKELQALN TEEAAEQRAE VDRMLSEDPW RAAKMIKGYM QQHNIPQREV VDVTGLNQSH LSQHLNKGTP MKTQKRAALY TWYVRKQREI LRQFNQTVQS SGNMTDKSSQ DQLLFLFPEF SQQSHGPGQS DDACSEPTNK KMRRNRFKWG PASQQILYQA YDRQKNPSKE EREALVEECN RAECLQRGVS PSKAHGLGSN LVTEVRVYNW FANRRKEEAF R (1-310 aa encoded by BC017714)

Target Information

Hepatocyte Nuclear Factor 1 (HNF1, Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor) is a member of the homeodomain-containing superfamily of transcription factors. It is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. HNF1has been shown to function in nephron development and regulates development of the embryonic pancreas. Mutations in the gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of the gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion.

Molecular cell

Battistello E,Hixon KA,Comstock DE,Collings CK,Chen X,Rodriguez Hernaez J,Lee S,Cervantes KS,Hinkley MM,Ntatsoulis K,Cesarano A,Hockemeyer K,Haining WN,Witkowski MT,Qi J,Tsirigos A,Perna F,Aifantis I,Kadoch C

Thu Apr 20 00:00:00 UTC 2023

Loss of Arid1a and Pten in Pancreatic Ductal Cells Induces Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway.

Gastroenterology

Fukunaga Y,Fukuda A,Omatsu M,Namikawa M,Sono M,Masuda T,Araki O,Nagao M,Yoshikawa T,Ogawa S,Hiramatsu Y,Muta Y,Tsuda M,Maruno T,Nakanishi Y,Ferrer J,Tsuruyama T,Masui T,Hatano E,Seno H

Mon Aug 01 00:00:00 UTC 2022

The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease.

Journal of experimental & clinical cancer research : CR

Cigliano A,Zhang S,Ribback S,Steinmann S,Sini M,Ament CE,Utpatel K,Song X,Wang J,Pilo MG,Berger F,Wang H,Tao J,Li X,Pes GM,Mancarella S,Giannelli G,Dombrowski F,Evert M,Calvisi DF,Chen X,Evert K

Fri Jun 03 00:00:00 UTC 2022

Recombinant protein Schistosoma japonicum-derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis.

World journal of gastroenterology

Zhang LC,Wu XY,Yang RB,Chen F,Liu JH,Hu YY,Wu ZD,Wang LF,Sun X

Tue Dec 14 00:00:00 UTC 2021

Computational and functional analyses of T2D GWAS SNPs for transcription factor binding.

Biochemical and biophysical research communications

Cheng M,Huang X,Zhang M,Huang Q

Thu Mar 12 00:00:00 UTC 2020

HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.

Journal of cellular and molecular medicine

Lu W,Sun J,Zhou H,Wang F,Zhao C,Li K,Fan C,Ding G,Wang J

Tue Dec 01 00:00:00 UTC 2020

HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.

Journal of cellular and molecular medicine

Lu W,Sun J,Zhou H,Wang F,Zhao C,Li K,Fan C,Ding G,Wang J

Tue Dec 01 00:00:00 UTC 2020

HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.

Oncogene

Wang J,He C,Gao P,Wang S,Lv R,Zhou H,Zhou Q,Zhang K,Sun J,Fan C,Ding G,Lan F

Sat Feb 01 00:00:00 UTC 2020

HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.

Oncogene

Wang J,He C,Gao P,Wang S,Lv R,Zhou H,Zhou Q,Zhang K,Sun J,Fan C,Ding G,Lan F

Sat Feb 01 00:00:00 UTC 2020

The nuclear receptor Shp regulates morphine withdrawal syndrome via modulation of Ugt2b expression in mice.

Biochemical pharmacology

Chen M,Guo L,Dong D,Yu F,Zhang T,Wu B

Fri Mar 01 00:00:00 UTC 2019

miR-194 regulates the proliferation and migration via targeting Hnf1β in mouse metanephric mesenchyme cells.

In vitro cellular & developmental biology. Animal

Liu Y,Hu Y,Ni D,Liu J,Xia H,Xu L,Zhou Q,Xie Y

Thu Aug 01 00:00:00 UTC 2019

Definitive endoderm differentiation is promoted in suspension cultured human iPS-derived spheroids more than in adherent cells.

The International journal of developmental biology

Yabe SG,Nishida J,Fukuda S,Takeda F,Nashiro K,Ibuki M,Okochi H

Fri Jan 24 00:00:00 UTC 2020

Definitive endoderm differentiation is promoted in suspension cultured human iPS-derived spheroids more than in adherent cells.

The International journal of developmental biology

Yabe SG,Nishida J,Fukuda S,Takeda F,Nashiro K,Ibuki M,Okochi H

Fri Jan 24 00:00:00 UTC 2020

Expression of mutant mRNA and protein in pancreatic cells derived from MODY3- iPS cells.

PloS one

Yabe SG,Nishida J,Fukuda S,Takeda F,Nasiro K,Yasuda K,Iwasaki N,Okochi H

Wed Jan 29 00:00:00 UTC 2020

Tumor-suppressive activity of Hnf1β in Wilms' tumor.

Bioscience, biotechnology, and biochemistry

Liu Y,Kanyomse Q,Xie Y

Fri Nov 01 00:00:00 UTC 2019

Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.

Scientific reports

Lewis PL,Su J,Yan M,Meng F,Glaser SS,Alpini GD,Green RM,Sosa-Pineda B,Shah RN

Wed Aug 15 00:00:00 UTC 2018

Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.

Scientific reports

Lewis PL,Su J,Yan M,Meng F,Glaser SS,Alpini GD,Green RM,Sosa-Pineda B,Shah RN

Wed Aug 15 00:00:00 UTC 2018

ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.

Scientific reports

Drosos Y,Escobar D,Chiang MY,Roys K,Valentine V,Valentine MB,Rehg JE,Sahai V,Begley LA,Ye J,Paul L,McKinnon PJ,Sosa-Pineda B

Mon Sep 11 00:00:00 UTC 2017

ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.

Scientific reports

Drosos Y,Escobar D,Chiang MY,Roys K,Valentine V,Valentine MB,Rehg JE,Sahai V,Begley LA,Ye J,Paul L,McKinnon PJ,Sosa-Pineda B

Mon Sep 11 00:00:00 UTC 2017

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

eLife

Nikolić MZ,Caritg O,Jeng Q,Johnson JA,Sun D,Howell KJ,Brady JL,Laresgoiti U,Allen G,Butler R,Zilbauer M,Giangreco A,Rawlins EL

Fri Jun 30 00:00:00 UTC 2017

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

eLife

Nikolić MZ,Caritg O,Jeng Q,Johnson JA,Sun D,Howell KJ,Brady JL,Laresgoiti U,Allen G,Butler R,Zilbauer M,Giangreco A,Rawlins EL

Fri Jun 30 00:00:00 UTC 2017

Molecular Mechanism Underlying the Suppression of CPB2 Expression Caused by Persistent Hepatitis C Virus RNA Replication.

Acta medica Okayama

Sejima H,Satoh S,Dansako H,Honda M,Kaneko S,Ikeda M,Kato N

Tue Jan 03 00:00:00 UTC 2017

Hepatocyte Nuclear Factor-1β Induces Redifferentiation of Dedifferentiated Tubular Epithelial Cells.

PloS one

Omata M,Doke Y,Yamada C,Kawashima K,Sho R,Enomoto K,Furuya M,Inomata N

Mon Jul 10 00:00:00 UTC 2017

Hepatocyte Nuclear Factor-1β Induces Redifferentiation of Dedifferentiated Tubular Epithelial Cells.

PloS one

Omata M,Doke Y,Yamada C,Kawashima K,Sho R,Enomoto K,Furuya M,Inomata N

Mon Jul 10 00:00:00 UTC 2017

Hepatocyte Nuclear Factor-1β Induces Redifferentiation of Dedifferentiated Tubular Epithelial Cells.

PloS one

Omata M,Doke Y,Yamada C,Kawashima K,Sho R,Enomoto K,Furuya M,Inomata N

Mon Jul 10 00:00:00 UTC 2017

Hepatocyte Nuclear Factor-1β Induces Redifferentiation of Dedifferentiated Tubular Epithelial Cells.

PloS one

Omata M,Doke Y,Yamada C,Kawashima K,Sho R,Enomoto K,Furuya M,Inomata N

Mon Jul 10 00:00:00 UTC 2017

Does clear cell carcinoma of stomach exist? Clinicopathological and prognostic significance of clear cell changes in gastric adenocarcinomas.

Histopathology

Kim JY,Park DY,Kim GH,Jeon TY,Lauwers GY

Tue Jul 01 00:00:00 UTC 2014

Does clear cell carcinoma of stomach exist? Clinicopathological and prognostic significance of clear cell changes in gastric adenocarcinomas.

Histopathology

Kim JY,Park DY,Kim GH,Jeon TY,Lauwers GY

Tue Jul 01 00:00:00 UTC 2014

Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.

Development (Cambridge, England)

Seth A,Ye J,Yu N,Guez F,Bedford DC,Neale GA,Cordi S,Brindle PK,Lemaigre FP,Kaestner KH,Sosa-Pineda B

Sat Feb 01 00:00:00 UTC 2014

Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.

Development (Cambridge, England)

Seth A,Ye J,Yu N,Guez F,Bedford DC,Neale GA,Cordi S,Brindle PK,Lemaigre FP,Kaestner KH,Sosa-Pineda B

Sat Feb 01 00:00:00 UTC 2014

Hepatocyte nuclear factor 1β is a novel prognostic marker independent of the Milan criteria in transplantable hepatocellular carcinoma: a retrospective analysis based on tissue microarrays.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

Shim JH,Lee HC,Han S,Kang HJ,Yu E,Lee SG

Fri Mar 01 00:00:00 UTC 2013

Hepatocyte nuclear factor 1β is a novel prognostic marker independent of the Milan criteria in transplantable hepatocellular carcinoma: a retrospective analysis based on tissue microarrays.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

Shim JH,Lee HC,Han S,Kang HJ,Yu E,Lee SG

Fri Mar 01 00:00:00 UTC 2013

Ovarian cancers arising from endometriosis: a microenvironmental biomarker study including ER, HNF1ß, p53, PTEN, BAF250a, and COX-2.

Journal of the Chinese Medical Association : JCMA

Lai CR,Hsu CY,Chen YJ,Yen MS,Chao KC,Li AF

Fri Nov 01 00:00:00 UTC 2013

Ovarian cancers arising from endometriosis: a microenvironmental biomarker study including ER, HNF1ß, p53, PTEN, BAF250a, and COX-2.

Journal of the Chinese Medical Association : JCMA

Lai CR,Hsu CY,Chen YJ,Yen MS,Chao KC,Li AF

Fri Nov 01 00:00:00 UTC 2013

Clear cell carcinomas of the ovary: a multi-institutional study of 129 cases in Korea with prognostic significance of Emi1 and Galectin-3.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Min KW,Park MH,Hong SR,Lee H,Kwon SY,Hong SH,Joo HJ,Park IA,An HJ,Suh KS,Oh HK,Yoo CW,Kim MJ,Chang HK,Jun SY,Yoon HK,Chang ED,Kim DW,Kim I

Tue Jan 01 00:00:00 UTC 2013

Clear cell carcinomas of the ovary: a multi-institutional study of 129 cases in Korea with prognostic significance of Emi1 and Galectin-3.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Min KW,Park MH,Hong SR,Lee H,Kwon SY,Hong SH,Joo HJ,Park IA,An HJ,Suh KS,Oh HK,Yoo CW,Kim MJ,Chang HK,Jun SY,Yoon HK,Chang ED,Kim DW,Kim I

Tue Jan 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Hepatocyte nuclear factor 1-beta; hepatocyte nuclear factor-1 beta; HNF-1-beta; HNF-1B; HNF1 beta A; Homeoprotein LFB3; TCF-2; Transcription factor 2; Transcription factor 2 hepatic; Transcription factor 2 hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2, hepatic; Transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; Variant hepatic nuclear factor 1; vHNF1

Gene Aliases: FJHN; HNF-1-beta; HNF-1B; HNF-1Beta; HNF1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1

UniProt ID: (Human) P35680, (Rat) P23899, (Mouse) P27889

Entrez Gene ID: (Human) 6928, (Rat) 25640, (Mouse) 21410

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding core promoter proximal region DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding protein complex binding protein homodimerization activity protein heterodimerization activity DNA binding sequence-specific DNA binding transcription regulatory region DNA binding DNA-binding transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endodermal cell fate specification kidney development inner cell mass cell differentiation transcription, DNA-templated Notch signaling pathway response to glucose anterior/posterior pattern specification response to organic cyclic compound insulin secretion regulation of Wnt signaling pathway hindbrain development circadian regulation of gene expression regulation of pronephros size pronephric nephron tubule development response to drug regulation of endodermal cell fate specification positive regulation of transcription, DNA-templated embryonic digestive tract morphogenesis branching morphogenesis of an epithelial tube pronephros development epithelial cell proliferation positive regulation of transcription initiation from RNA polymerase II promoter ureteric bud elongation hepatoblast differentiation negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis protein-DNA complex assembly hepatocyte differentiation regulation of branch elongation involved in ureteric bud branching mesonephric duct formation negative regulation of mesenchymal cell apoptotic process involved in metanephros development liver development transcription from RNA polymerase II promoter endoderm development response to carbohydrate positive regulation of gene expression endocrine pancreas development negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter embryonic morphogenesis genitalia development epithelium development kidney morphogenesis mesonephric tubule development nephric duct development mesonephric duct development nephric duct formation endoderm formation regulation of transcription, DNA-templated

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HNF1B Polyclonal Antibody

HNF1B Antibody (12533-1-AP) in ICC/IF

Product Details

12533-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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