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Invitrogen

HNF4A Monoclonal Antibody (F.674.9)

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2 Published Figures

3 References

View all (45) HNF4A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

HNF4A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

HNF4A Antibody (MA5-14891) in ICC/IF

Immunofluorescence analysis of HNF4A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HNF4A Rabbit Monoclonal Antibody(Product # MA5-14891) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

HNF4A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HNF4A Antibody in Immunohistochemistry (IHC)

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HNF4A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of HNF4 was observed in HepG2, Caco-2 and A549 in comparison to HCT-116 using product (Product # MA5-14891 ) in western blot. {RE}

Product Details

MA5-14891

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:6000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.674.9

Immunogen

Synthetic peptide corresponding to the sequence of human HNF4a

Conjugate

Unconjugated

Form

Liquid

Concentration

52 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980013

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic Liver Injury Induces Conversion of Biliary Epithelial Cells into Hepatocytes.

Cell stem cell

Deng X,Zhang X,Li W,Feng RX,Li L,Yi GR,Zhang XN,Yin C,Yu HY,Zhang JP,Lu B,Hui L,Xie WF

MA5-14891 was used in Immunofluorescence in immunohistochemistry on frozen tissues to provide further support for hepatocyte regeneration from BECs without genetic interventions and show their cellular plasticity during severe liver injury.

Thu Jul 05 00:00:00 UTC 2018

Chronic Liver Injury Induces Conversion of Biliary Epithelial Cells into Hepatocytes.

Cell stem cell

Deng X,Zhang X,Li W,Feng RX,Li L,Yi GR,Zhang XN,Yin C,Yu HY,Zhang JP,Lu B,Hui L,Xie WF

Thu Jul 05 00:00:00 UTC 2018

Activation of liver stromal cells is associated with male-biased liver tumor initiation in xmrk and Myc transgenic zebrafish.

Scientific reports

Yang Q,Yan C,Gong Z

Published figure using HNF4A monoclonal antibody (Product # MA5-14891) in Immunofluorescence

Mon Sep 04 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4-alpha; HNF4alpha10/11/12; Nuclear receptor subfamily 2 group A member 1; TCF-14; Transcription factor 14; Transcription factor HNF-4

Gene Aliases: FRTS4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14

UniProt ID: (Human) P41235

Entrez Gene ID: (Human) 3172

Function(s)

fatty-acyl-CoA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding fatty acid binding protein binding zinc ion binding palmitoyl-CoA hydrolase activity protein homodimerization activity transcription regulatory region DNA binding C4 zinc finger nuclear receptor

Process(es)

regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter ornithine metabolic process lipid metabolic process acyl-CoA metabolic process xenobiotic metabolic process establishment of tissue polarity sex differentiation blood coagulation negative regulation of cell proliferation response to glucose regulation of gastrulation regulation of lipid metabolic process signal transduction involved in regulation of gene expression cell differentiation negative regulation of cell growth intracellular receptor signaling pathway regulation of microvillus assembly negative regulation of protein import into nucleus, translocation response to drug negative regulation of tyrosine phosphorylation of Stat5 protein glucose homeostasis steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity cell-cell junction organization positive regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter regulation of insulin secretion response to cAMP lipid homeostasis phospholipid homeostasis SMAD protein signal transduction triglyceride homeostasis negative regulation of activation of JAK2 kinase activity positive regulation of cholesterol homeostasis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HNF4A Monoclonal Antibody (F.674.9)

HNF4A Antibody (MA5-14891) in ICC/IF

Product Details

MA5-14891

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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HNF4A Monoclonal Antibody (F.674.9)

HNF4A Antibody (MA5-14891) in ICC/IF

Product Details

MA5-14891

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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