Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
FIGURE: 1 / 6
HNF4A Antibody (417700) in ICC/IF
Immunofluorescence analysis of HNF4A was performed using 70% confluent log phase HepG2 and HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-HNF4A Monoclonal Antibody (Product # 417700) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
417700
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
H1415
Immunogen
Baculovirus-expressed recombinant human HNF4-alpha (393-461 aa)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Wet ice
RRID
AB_2532197
Product Specific Information
This antibody specifically recognizes human HNF4 alpha 1, 2, 4, 5, 7 ,8 and cross reacts with mouse and rat HNF4 alpha 1, 2, 4, 5, 7 ,8.
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support