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Invitrogen

HOMER3 Polyclonal Antibody

5个已发表图片

6 篇参考文献

查看其他6个HOMER3抗体

问题与答案

问题与答案

Application

HOMER3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

HOMER3 Antibody (PA5-59383) in ICC/IF

Immunofluorecent analysis of HOMER3 in human cell line A-431 using HOMER3 Polyclonal Antibody (Product # PA5-59383). Staining shows localization to plasma membrane and cytosol.

HOMER3 Antibody in Immunocytochemistry (ICC/IF)

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HOMER3 Antibody in Immunohistochemistry (IHC)

HOMER3 Antibody in Immunohistochemistry (IHC)

HOMER3 Antibody in Immunohistochemistry (IHC)

HOMER3 Antibody in Western Blot, Immunocytochemistry, Flow Cytometry, Immunoprecipitation (WB, ICC/IF, Flow, IP)

HOMER3 Antibody in Immunohistochemistry (IHC)

HOMER3 Antibody in Immunohistochemistry (IHC)

HOMER3 Antibody in Western Blot, Flow Cytometry (WB, Flow)

HOMER3 Antibody in Western Blot (WB)

HOMER3 Antibody

HOMER3 Antibody

Product Image Gallery

HOMER3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorecent analysis of HOMER3 in human cell line A-431 using HOMER3 Polyclonal Antibody (Product # PA5-59383). Staining shows localization to plasma membrane and cytosol.

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HOMER3 in human pancreas using HOMER3 Polyclonal Antibody (Product # PA5-59383) shows no positivity in exocrine glandular cells.

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HOMER3 in human spleen using HOMER3 Polyclonal Antibody (Product # PA5-59383) shows strong cytoplasmic positivity in red pulp.

HOMER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HOMER3 in human liver using HOMER3 Polyclonal Antibody (Product # PA5-59383) shows no positivity in hepatocytes.

HOMER3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of HOMER3 in human spleen and pancreas tissues using HOMER3 Polyclonal Antibody (Product # PA5-59383). Corresponding HOMER3 RNA-seq data are presented for the same tissues.

HOMER3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of HOMER3 in human pancreas using HOMER3 Polyclonal Antibody (Product # PA5-59383) shows low expression as expected.

HOMER3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of HOMER3 in human spleen using HOMER3 Polyclonal Antibody (Product # PA5-59383) shows high expression.

HOMER3 Antibody in Western Blot, Immunocytochemistry, Flow Cytometry, Immunoprecipitation (WB, ICC/IF, Flow, IP) — Fig. 3 A ) HOMER3 is expressed by the majority of 5637 and T24 cells (> 90%) showing mostly intracellular origin, whereas a small subpopulation also exhibits HOMER3 at the cell surface (3-5%). The left panel shows the expression of HOMER3 at the cell surface in a small subset of T24 cells (3-5%) and a massive expression of total HOMER3 (> 90% of the cells). The graph histogram at right highlights that 5637 and T24 cells presented the same HOMER3 phenotype. Experiments assessing HOMER3 at the cell surface were conducted in cells showing non-permeabilized membranes and without incorporation of propidium iodide. The analysis of permeabilized cells showed HOMER3 total levels. Results are the average of three independent replicates **** p < 0.0001 (student t-test). B ) Immunofluorescence microscopy for HOMER3 (green) in non-permeabilized and permeabilized cells highlighting HOMER3 at the cell membrane (left panel). C ) Immunofluorescence microscopy for HOMER3 (green) at the cell surface of non-permeabilized cells, as confirmed by a uniform expression of CellMask (orange) and the absence of signal for propidium iodine (red). Propidium iodine staining was used as cell death control for exclusion of cells with compromised plasma membranes (lower panel). In addition to PI, dead T24 cells also stained more intensely for cell mask than live cells due to dye diffusion into the intracellular space. D ) Expression of HOMER3 in whole cell extracts, HOMER3 immunoprecipitates (IP) and corresp

HOMER3 Antibody in Immunohistochemistry (IHC) — Fig. 7 Sialylated HOMER3 glycoforms can be found at the cell-surface of bladder cancer cells and display significant inter-patient structural variability. A ) HOMER3 at the cell membrane co-localizes in the same tumour area with sialylated Tn and T antigens in bladder cancer. According to immunohistochemistry, HOMER3 was diffusely expressed in wide tumour areas, both at the cytoplasm and cell membranes. These areas co-localized with very high STn and/or ST expressions areas. B ) Immunofluorescence microscopy highlighting the co-localization (in white) of HOMER3 (violet) with sialylated Tn and T antigens (green) at the cell membrane of bladder cancer cells. HOMER3 was detected in both cytoplasm and cell membrane of a high number of cancer cells in invasive tumours. The tumours were also screened for the Tn and T antigens and their sialylated counterparts STn and ST. Tumours were negative for neutral glycans but showed high sialospecies, in accordance with immunohistochemistry analysis in panel A. C ) MS/MS HOMER3 glycopeptide with cancer-associated glycans isolated from an invasive bladder tumour. Briefly, invasive tumours showing no Tn and T antigens were elected for this analysis. Glycoproteins were extracted from these tumours and digested with neuraminidase to expose Tn and T antigens derived from their sialylated counterparts. VVA and PNA lectins were then used to pulldown glycoproteins carrying these glycans for downstream analysis by nanoLC-MS/MS. Peptide and glycopepti

HOMER3 Antibody in Immunohistochemistry (IHC) — Fig. 8 HOMER3 and STn are not co-expressed at the cell membrane in relevant human healthy tissues (thyroid, liver, gallbladder, testis, lung, stomach, pancreas, colon, small intestine, appendix). HOMER3 was not detected in these tissues, except for few cells in the submucosa of digestive organs as well as in thyroid follicular cells, all of which showing weak/moderate cytoplasmic staining. HOMER3 was also detected in respiratory tract secretions, suggesting potential translocation of the protein to the extracellular space, warranting confirmation in future studies. Moreover, HOMER3 was not found at the cell surface of healthy cells, contracting with observations for cancer cells. The STn antigens could only be found in mucinous secretions of the gastrointestinal and respiratory tracts, being also scarcely observed at the cell surface of cells facing the lumen of these organs. No evidence of co-localization between both antigens could be observed

HOMER3 Antibody in Western Blot, Flow Cytometry (WB, Flow) — Fig. 4 Hypoxia and glucose deprivation drive the accumulation of HOMER3 at the cell membrane. A ) Hypoxia and Glucose deprivation significantly increased the number of T24 and 5637 cells expressing HOMER3 at the cell surface. T24 flow cytometry analysis (included in the left panel as a representative example) and graph A (right panel) shows that the vast majority of T24 and 5637 cancer cells express HOMER3, which remains unchanged upon tremendous reduction in oxygen levels and glucose deprivation. On the other hand, this microenvironmental feature promoted a massive increase of cells (approximately 5-fold) showing HOMER3 at the cell surface. B ) Induction of HOMER3 upregulation and downregulation in T24 cells. Blots confirm the development of two HOMER3 knockdown (T24_HOMER3_KD1 and KD2; 30-50% decrease), one knockout model (T24_HOMER3_KO; total HOMER3 abrogation) and an HOMER3 knock-in (T24_HOMER3_KI; 60% increase compared with the control). C ) T24 overexpression is mainly observed at the cell surface after exposure to hypoxia and glucose deprivation. The percentage of cells expressing HOMER3 increases significantly in relation to the controls in both normoxia and hypoxia plus glucose deprivation; however, this increase is more pronounced under microenvironmental pressure. D ) HOMER3 downregulation or deletion leads to a massive decrease in the number of cells expressing the protein in normoxia, irrespectively of the sub-cellular localization, which is not compensated by ex

HOMER3 Antibody in Western Blot (WB) — Figure 8. RNA sequence analysis and phosphoproteomics analysis. A , B , Many oxidative phosphorylation-related genes in Complex I, Complex III, Complex IV, and Chemiosmosis are upregulated in Het PKC gamma -A24E mice in RNA sequencing. A , IPA showed that many ubiquinone oxidoreductase subunits and cytochrome c oxidase subunits are significantly upregulated in Het PKC gamma -A24E mice. y axis is log2 fold change. B , Red complexes are upregulated in Het PKC gamma -A24E mice, which locate in the mitochondrial membrane. C , Network analysis was performed by IPA of gene sets upregulated or downregulated in Het PKC gamma -A24E mice compared with Wt littermates. Green genes represent decreases. Red genes represent increases. Many genes related to Purkinje cell morphology are downregulated in Het PKC gamma -A24E mice. D , Volcano plot of phospho-proteomics analysis of Wt versus Homo PKC gamma -A24E mice ( n = 3). Lysates from 7-week-old Wt and PKC gamma -A24E mice were subjected to phosphoproteomic analysis by mass spectrometry. Differentially enriched phosphopeptides are shown in the volcano plot. x axis is log2 fold change, and y axis is p value. Rmdn3 and Homer3 are among the proteins with a significantly increased phosphorylation in PKC gamma -A24E mice. E , RNA sequence data show that Rmdn3 and Homer3 are upregulated in PKC gamma -A24E mice. F , Western blot analysis of Rmdn3 and Homer3 from organotypic slice cultures. Homer3 protein expression (Wt = 100.0%, n = 4; Het = 149.7

HOMER3 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of HOMER3 demonstrates antibody specificity. Immunohistochemical analysis of HOMER3 using anti-HOMER3 Polyclonal Antibody (Product # PA5-59383), shows significant staining of HOMER3 in human spleen and shows minimal or weak staining in human pancreas tissues. The relative expression levels of HOMER3 within each tissue is shown using RNA-Seq. {RE}

HOMER3 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of HOMER3 demonstrates antibody specificity. Immunohistochemical analysis of HOMER3 using anti-HOMER3 Polyclonal Antibody (Product # PA5-59383), shows significant staining of HOMER3 in spleen and shows minimal or weak staining in pancreas tissues. The relative expression levels of HOMER3 within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-59383

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,000-1:2,500

-

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

-

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human HOMER3. Recombinant protein control fragment (Product #RP-98314).

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2642508

产品详细信息

Immunogen sequence: ALVQTKDQEI QTLKSQTGGP REALEAAERE ETQQKVQDLE TRNAELEHQL RAMERSLEEA RAERERARAE VGRAAQLLDV RLFELSELRE GL

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 88%.

靶标信息

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A New Mouse Model Related to SCA14 Carrying a Pseudosubstrate Domain Mutation in PKCγ Shows Perturbed Purkinje Cell Maturation and Ataxic Motor Behavior.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Shimobayashi E,Kapfhammer JP

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Mar 03 00:00:00 UTC 2021

A New Mouse Model Related to SCA14 Carrying a Pseudosubstrate Domain Mutation in PKCγ Shows Perturbed Purkinje Cell Maturation and Ataxic Motor Behavior.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Shimobayashi E,Kapfhammer JP

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Mar 03 00:00:00 UTC 2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Journal of experimental & clinical cancer research : CR

Peixoto A,Ferreira D,Azevedo R,Freitas R,Fernandes E,Relvas-Santos M,Gaiteiro C,Soares J,Cotton S,Teixeira B,Paulo P,Lima L,Palmeira C,Martins G,Oliveira MJ,Silva AMN,Santos LL,Ferreira JA

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Jun 09 00:00:00 UTC 2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Journal of experimental & clinical cancer research : CR

Peixoto A,Ferreira D,Azevedo R,Freitas R,Fernandes E,Relvas-Santos M,Gaiteiro C,Soares J,Cotton S,Teixeira B,Paulo P,Lima L,Palmeira C,Martins G,Oliveira MJ,Silva AMN,Santos LL,Ferreira JA

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Jun 09 00:00:00 UTC 2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Journal of experimental & clinical cancer research : CR

Peixoto A,Ferreira D,Azevedo R,Freitas R,Fernandes E,Relvas-Santos M,Gaiteiro C,Soares J,Cotton S,Teixeira B,Paulo P,Lima L,Palmeira C,Martins G,Oliveira MJ,Silva AMN,Santos LL,Ferreira JA

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Jun 09 00:00:00 UTC 2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Journal of experimental & clinical cancer research : CR

Peixoto A,Ferreira D,Azevedo R,Freitas R,Fernandes E,Relvas-Santos M,Gaiteiro C,Soares J,Cotton S,Teixeira B,Paulo P,Lima L,Palmeira C,Martins G,Oliveira MJ,Silva AMN,Santos LL,Ferreira JA

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Jun 09 00:00:00 UTC 2021

Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

Journal of experimental & clinical cancer research : CR

Peixoto A,Ferreira D,Azevedo R,Freitas R,Fernandes E,Relvas-Santos M,Gaiteiro C,Soares J,Cotton S,Teixeira B,Paulo P,Lima L,Palmeira C,Martins G,Oliveira MJ,Silva AMN,Santos LL,Ferreira JA

Published figure using HOMER3 polyclonal antibody (Product # PA5-59383) in Western Blot

Wed Jun 09 00:00:00 UTC 2021

生物信息学

蛋白别名: homer homolog 3; Homer protein homolog 3; Homer, neuronal immediate early gene, 3; Homer-3

基因别名: HOMER-3; HOMER3; VESL3

UniProt ID: (Human) Q9NSC5

Entrez Gene ID: (Human) 9454

功能

protein binding protein C-terminus binding protein domain specific binding G-protein coupled glutamate receptor binding scaffold/adaptor protein

参与通路

1x1 image pixel for data collection